(A) Forty-seven primary fibroblast populations, from 43 unique anatomic sites, were obtained from arm (blue circles), leg (turquoise circles), trunk (pink circles), foreskin (yellow circles), and internal organs (red circles). The cells were derived from 20 different human autopsy or surgery donors identified by the letters A through T in parenthesis.

(B) Diversity of gene expression programs in 47 fibroblast populations. Each row represents a gene; each column represents a fibroblast population. The expression level of each gene is represented relative to the median value in all samples; expression levels above and below the global median are denoted by shades of red or green, respectively. The color scale encompasses a range from 32- to 0.03-fold relative to global median transcript level for each gene (+5 to −5 logs on log base 2 scale). Black represents the median expression value; gray represents missing data.

(C) Similarity in the global gene expression profiles of 47 fibroblast samples. We used unsupervised hierarchical clustering of 7,580 genes that were reliably measured in 70% of the samples and varied by 3-fold above the global median in at least five (approximately 10%) of the samples. Thirty-five of the 47 samples were placed in clusters composed predominantly of cells from the same anatomic origins (arm, leg, trunk, foreskin, or nondermal).