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News from Arabidopsis on the Meiotic Roles of Blap75/Rmi1 and Top3α

  • Charles I. White mail

    chwhite@univ-bpclermont.fr

    Affiliation: Génétique, Reproduction, et Développement, CNRS UMR 6247, Clermont Université, INSERM U931, Aubière Cedex, France

    X
  • Published: December 19, 2008
  • DOI: 10.1371/journal.pgen.1000306

Two articles published in this issue of PLoS Genetics present novel data concerning the members of a key regulator of genetic crossing-over. Working with the plant Arabidopsis thaliana, the authors of the two reports provide exciting new data and further understanding of the meiotic anti–crossing-over function of the Topoisomerase 3alpha (Top3α) and Blap75/Rmi1 proteins, and thus presumably that of the protein complex that contains these proteins and the RecQ-like helicase BLM.

The highly conserved RecQ-like helicase BLM, which is mutated in patients with Bloom syndrome, acts in a protein complex that can disassociate homologous recombination intermediates in vitro and in vivo (reviewed in [1][3]). The importance of this anti-recombination role is clearly shown by the elevated levels of genetic instability, mitotic recombination, and sister-chromatid exchanges in the somatic tissues of the cancer-prone Bloom syndrome patients. This complex, known as BTB in mammals and RTR in yeast, involves BLM and at least two other proteins: Top3α and Blap75/Rmi1. BLAP75/RMI1 is a highly conserved protein in eukaryotes originally identified through its interactions with the BLM [4],[5] and independently as Rmi1/Nce4 in yeast through its genetic interactions with BLM homolog Sgs1 [1],[6]. A fourth protein component of this complex, Rmi2, has recently been identified [7],[8], and it is likely that others will follow (discussed by [9]). It is proposed that the principal anti-recombinational role of this complex involves BLM helicase-driven migration of double Holliday junctions (dHJs) to form a hemi-catenane intermediate. The resolution of this structure by the action of a topoisomerase (Top3α) does not lead to the exchange of flanking DNA sequences, and thus BLM acts to avoid crossing-over [3], [10][12]. BLM also has affinity for DNA structures other than dHJ and clearly also plays other anti-recombination roles [13][15]. To add to these, a very recent report shows a pro-recombination role for Sgs1/BLM in resection of 5′-ended strands at DNA double-strand breaks [16].

What about A. thaliana, the subject of the two reports discussed here? Arabidopsis has a total of seven identified RecQ-like proteins, with RecQ4a being the strongest candidate for the Arabidopsis BLM/Sgs1 ortholog [17][19]. The accompanying papers report the identification of the Arabidopsis othologs of BLAP75/Rmi1 [20],[21] and Topo3α [21], as well as the characterization of the mitotic and meiotic phenotypes of the corresponding mutant plants. top3α mutant plants have severe developmental defects, are methyl methanesulfonate (MMS)–sensitive, and show elevated levels of mitotic recombination and mitotic chromosome abnormalities. Similar mitotic phenotypes are observed in recQ4a and blap75/rmi1 mutant plants, suggesting a functional interaction between RecQ4a and Top3α. This is further supported by the partial suppression of top3α developmental defects in double recQ4a/top3α mutants.

In most (studied) eukaryotes, homologous recombination that occurs during the first meiotic prophase ensures the proper segregation of homologous chromosomes (homologs) at the first meiotic division. These events are initiated by programmed double-strand breaks that generate broken DNA ends that invade homologous sequences on the homolog, a subset of which are processed to form dHJs. These must be resolved to permit the separation of homologs at the first meiotic anaphase, and the mode of this resolution determines whether or not the recombination is accompanied by physical exchange of chromosome arms of the homologs (crossing-over). The potential of crossing-over to cause genome reorganization (insertions, deletions, inversions, translocations) has led to the evolution of multiple controls of recombination.

It has long been recognized that the numbers and distribution of meiotic cross-overs are strictly regulated. In the last decade, the existence of cross-over and non–cross-over recombination pathways has been established, and many details of molecular mechanisms elucidated [22][28]. In this context lies the importance of the characterization of the essential meiotic anti–crossing-over role of the BTB/RTR complex in Arabidopsis by the Grelon and Puchta groups, reported in this issue of PLoS Genetics [20],[21].

These reports show that Arabidopsis blap75/rmi1 and top3α mutants are capable of full chromosome synapsis, resulting in normal pachytene figures. The structure of the synaptonemal complex at pachytene was verified by staining of blap75 mutant meioses with antisera against Asy1 and Zyp1, two synaptonemal complex proteins, and proper chromosome pairing was shown by fluorescence in situ hybridization (FISH) [20]. Staining with antiserum against Dmc1, a marker for early meiotic recombination intermediates, also shows normal numbers and timing of foci. Although these immunological and FISH analyses haven't been carried out for top3α mutants, the DAPI-stained pachytene figures of top3α present the same (normal) aspect as those of the blap75 mutants. Epistasis analyses confirm that Blap75/Rmi1 acts downstream of Spo11 (DNA cleavage/recombination initiation), Rad51, and Mnd1 (homolog invasion). It thus appears that early steps of meiosis, up to homolog pairing and synaptonemal complex formation, occur normally in the absence of Blap75/Rmi1 and Top3α in Arabidopsis. However, aberrant diakinesis and interlocked metaphase I figures follow, and chromosomes fragment at snaphase I. The interlocked bivalents observed at diakinesis and metaphase I may be due to the presence of the unresolved recombination intermediates, including those between more than two chromatids that are seen in yeast [29],[30]. The fragmentation of univalent chromosomes at meiotic anaphase I in double dmc1/blap75 mutants might also suggest such interchomatid linkages (Arabidopsis dmc1 mutants have asynaptic meiosis [31]), although other explanations, such as the existence of unrepaired chromatid breaks, are equally likely. Interestingly, no later meiotic stages (second division) were seen in either study, implying meiotic arrest at the end of the first division. This phenotype contrasts strikingly with that seen in many Arabidopsis recombination mutants, such as rad50 or rad51, which complete the two meiotic divisions, notwithstanding severe chromosome fragmentation at leptotene [32],[33]. The dependence of this meiosis I arrest upon the presence of paired chromosomes is confirmed by the completion of the two meiotic divisions in double dmc1/blap75 mutants. Homologous chromosome recognition and synapsis through recombination thus progresses to bivalent formation in blap75/rmi1 mutants, even in the absence of the ZMM proteins Mer3 and Msh5—notwithstanding the fact that they are required for the major meiotic cross-over pathway in Arabidopsis [34][36]. The bivalents of blap75/rmi1 mutants are, however, interlinked and unable to separate properly, finally fragmenting at anaphase I.

Blap75/Rmi1 and Top3α are thus needed for resolution of recombination intermediates that form between both sister chromatids and homologs, and are essential for separation of bivalents and meiosis I chromosomal disjunction in Arabidopsis. An essential meiosis I role is also seen in yeast top3 and rmi1 mutants [1],[37]. However, Arabidopsis recQ4a mutants have apparently normal meiosis and are fertile, as are mouse BLM−/− mutants, and yeast sgs1 mutants show only minor defects in meiosis [38],[40]. Thus, Blap75/Rmi1 and Top3α appear to have meiotic functions that are independent of RecQ4a. The relative high fertility of yeast sgs1 mutants may be explained by findings that Sgs1 and the Mus81/Mms4 nuclease can partially substitute for each other [29],[30]. The co-lethality of recQ4a and mus81 in Arabidopsis [39] is suggestive of a similar situation in mitosis in yeast, although their relation in meiosis remains to be determined.

These findings lead to the question of whether the critical meiotic function of Blap75/Rmi1 and Top3α in resolving recombination intermediates is performed by these two proteins alone, or whether they perform this function in complex with another helicase. Other unanswered questions concern the nature of the meiotic joint molecule intermediates, the resolution of which requires Blap75/Rmi1 and Top3α in Arabidopsis. Does the absence of these proteins lead to an excess of normal dHJs, overwhelming the capacity of other HJ resolvase(s), or are these aberrant, unresolvable structures? The Mus81 nuclease acts in an interference-insensitive cross-over pathway in Arabidopsis meiosis [41], but clearly it cannot complement the absence of Blap75/Rmi1 or Top3α—what role does it play in recombination intermediate metabolism in Arabidopsis meiosis?

In addition to their importance for the understanding of recombination and meiosis in plants, these results extend the known meiotic activities of this complex—demonstrating and clearly placing its essential role in the separation of synapsed chromosomes at the first meiotic division.

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