Research Article

Numt-Mediated Double-Strand Break Repair Mitigates Deletions during Primate Genome Evolution

  • Einat Hazkani-Covo mail,

    Affiliation: National Evolutionary Synthesis Center, Durham, North Carolina, United States of America

  • Shay Covo

    Affiliation: Laboratory of Molecular Genetics, Chromosome Stability Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of Amercia

  • Published: October 24, 2008
  • DOI: 10.1371/journal.pgen.1000237


Non-homologous end joining (NHEJ) is the major mechanism of double-strand break repair (DSBR) in mammalian cells. NHEJ has traditionally been inferred from experimental systems involving induced double strand breaks (DSBs). Whether or not the spectrum of repair events observed in experimental NHEJ reflects the repair of natural breaks by NHEJ during chromosomal evolution is an unresolved issue. In primate phylogeny, nuclear DNA sequences of mitochondrial origin, numts, are inserted into naturally occurring chromosomal breaks via NHEJ. Thus, numt integration sites harbor evidence for the mechanisms that act on the genome over evolutionary timescales. We have identified 35 and 55 lineage-specific numts in the human and chimpanzee genomes, respectively, using the rhesus monkey genome as an outgroup. One hundred and fifty two numt-chromosome fusion points were classified based on their repair patterns. Repair involving microhomology and repair leading to nucleotide additions were detected. These repair patterns are within the experimentally determined spectrum of classical NHEJ, suggesting that information from experimental systems is representative of broader genetic loci and end configurations. However, in incompatible DSBR events, small deletions always occur, whereas in 54% of numt integration events examined, no deletions were detected. Numts show a statistically significant reduction in deletion frequency, even in comparison to DSBR involving filler DNA. Therefore, numts show a unique mechanism of integration via NHEJ. Since the deletion frequency during numt insertion is low, native overhangs of chromosome breaks are preserved, allowing us to determine that 24% of the analyzed breaks are cohesive with overhangs of up to 11 bases. These data represent, to the best of our knowledge, the most comprehensive description of the structure of naturally occurring DSBs. We suggest a model in which the sealing of DSBs by numts, and probably by other filler DNA, prevents nuclear processing of DSBs that could result in deleterious repair.

Author Summary

Changes to DNA sequence are the major source of variation in evolution. Those changes often arise from damage to DNA that is repaired in a way that fails to restore the original sequence. One type of DNA damage is a chromosomal double-strand break. Such breaks are mostly studied experimentally in model systems, because naturally occurring chromosomal breaks are hard to follow. Here, we used an evolutionary approach to study the repair of naturally occurring chromosomal breaks. Throughout evolutionary history, fragments of the mitochondrial genome, known as numts (nuclear sequences of mitochondrial origin), have been inserted into the nuclear genome. Numts are passively captured into random chromosomal breaks, leaving sequence traces in genomes. Humans and chimpanzees share a recent common ancestor and their genomes share high sequence similarity; therefore, their species-specific numts can be used to follow both some of the break structure and repair mechanisms. Comparing naturally occurring break and repair patterns with experimental repair patterns identified similarities but also highlighted a clear difference. Experimental breaks usually involve deletions, while deletions were significantly less frequent in the numt based repair system. We propose that extra-chromosomal DNA sequences, like numts, play a role in maintaining genome integrity by protecting naturally occurring chromosomal breaks from further deleterious processing.


The major mechanism of double-strand break repair (DSBR) in mammalian cells involves the religation of the two broken ends of the damaged chromosome by DNA Ligase IV, a process known as classical non-homologous end joining (NHEJ) [1],[2]. The hallmarks of NHEJ activity are, first, the tendency of the DNA termini to form base-pair complements, also known as microhomology-mediated repair, and second, that it is an error prone process in which nucleotides are often deleted or added. The mechanisms of chromosomal NHEJ repair of double strand breaks (DSBs) have been studied in great detail in two experimental model systems: V(D)J recombination [2],[3] and I-SceI-induced DSBs [4][7]. In each case, the repair of specific DSB end configurations generated by endonucleases at specific loci has been studied. It is unclear if the same repair pattern is shared between experimental and naturally occurring breaks, as the latter are much more diverse in respect to their genomic locations and break configurations [8],[9]. Inaccurate repair of naturally occurring breaks has driven chromosome evolution by introducing structural changes [10]. Whether or not the spectrum of NHEJ repair events observed in experimental systems is a reflection of repair of DSBs during chromosomal evolution is an unresolved issue.

Evidence has accumulated to suggest that extra-chromosomal DNA (also known as filler DNA) is captured into DSB repair sites via NHEJ [5], [11][17]. If true, then evidence for DSBR should be preserved in genomes and identifiable in genome comparisons spanning short evolutionary times. Analyzing these genomic records of DSBR will shed light on the processes of DSBR and chromosome evolution.

We examined mitochondrial sequences that were inserted into the nuclear genomes of human and chimpanzee after the divergence of the two species about 5–6 Myr ago [16],[18]. Nuclear sequences of mitochondrial origin [numts, 19] have been identified in numerous sites throughout nuclear genomes [20][22] in species ranging from yeast to plants and humans [23],[24] based on their sequence similarity to mitochondrial DNA. Numts are randomly distributed among the chromosomes with no apparent integration hotspots [18],[25]. However, it was suggested that numt are common in introns and near repeats [16],[26]. In humans, numt sizes range from tens of bases to an entire mitochondrion (16 kb) and represent 430 kb of the genome [18],[25]. Numts appear on all chromosomes and integration of numts into genes has also been associated with diseases [27],[28]. A deluge of mitochondrial DNA has been steadily transferred to the nucleus since the origin of the mitochondria from the α-proteobacterial endosymbiotic ancestor [29]. All mammalian numts studied to date are considered “dead-on-arrival” pseudogenes [30], but evidence for functional numts has been reported in species including plants, yeasts and flies [31],[32].

Numts have unique characteristics that make them especially suitable to the study of evolutionary signatures of DSBR: i) they are unable to actively integrate into the genome (in contrast to LINEs and SINEs [33]), and instead, are captured into the nuclear genome via an NHEJ mechanism [15][17],[34]; ii) they possess no intrinsic ability to transpose after insertion by NHEJ, and thus are a stable marker of a repaired DSB; and iii) since numts are derived from the mitochondrial genome, they are easily identified and distinguished from the nuclear DNA by sequence analysis. Thus, the numt-chromosome borders are well-defined and sites of fusion (fusion points) can be determined with single-nucleotide resolution.

We describe a comprehensive molecular analysis of numt fusion points throughout the human and chimpanzee genomes. Some of the repair patterns observed in experimental systems are detected during chromosome evolution in various genetic loci and natural DSB configurations. Surprisingly, numt-mediated NHEJ involves fewer deletions in comparison with experimental NHEJ, whether different types of filler DNA were present or not. We suggest a model according in which filler DNAs may play a role in protecting genome integrity from deleterious processing of DSBs.


Comprehensive Analysis of numt Integration through Hominoid Evolution Supports NHEJ-Mediated Insertion Mechanism

Human- and chimpanzee-specific numts were identified based on the genome alignment of human and chimpanzee using rhesus monkey as an outgroup (Figure 1). In total, 55 chimpanzee-specific numts and 35 human-specific numts were identified (see Methods).


Figure 1. Phylogenetic tree of human, chimpanzee and rhesus monkey showing new numt insertions used for DSBR analysis.

Recent human and chimpanzee numt insertions according to triangulate classification are shown on each branch. The numt polymorphic variants [16] (NP, in gray) are optional in our analysis. Human and chimpanzee (HC) common ancestor is indicated.


Two possibilities pertaining to the origin of numt previously were considered for the entire numt repertoire in the human genome: independent insertion from the mitochondria and genomic duplication subsequent to the insertion [20],[21]. Recent numts, however, generally are considered to have been inserted independently from the mitochondria via NHEJ based on experimental studies, lack of homologies in the flanking regions, and the appearance of NHEJ hallmarks in the fusion points [15][17],[34]. We tested this hypothesis in this study. Numts inserted from the mitochondria potentially could be inserted by homologous recombination between chromosomal and mitochondrial DNA. If this is true, numt-flanking regions should show sufficient sequence identity with mitochondrial DNA. To test this possibility, each numt identified in the study, along with 100 bases of up- and down-stream flanking chromosomal sequences, was BLASTed against the mitochondria (see Methods). We could not find sequence identities between mitochondria and chromosomal DNA that include more than seven bp from the numt, thus, no evidence was found for insertion by homologous recombination.

Numts have no self-replicating mechanism, therefore, the prediction is that numt duplication is expected to be part of a larger segmental duplication [20],[35],[36]. In this case, numt is predicted to insert through non-allelic homologous recombination between chromosomal DNA and preexisting nuclear numt and should be characterized by DNA sequence homology that extends beyond the numt [20]. To test this possibility, each numt identified in the study, along with 100 bases of up- and down-stream flanking chromosomal sequence, was BLASTed against the nuclear genome. None of the numts and flanking regions showed sequence identity with the genomic target to account for non-allelic homologous recombination (see Methods). We also looked for numts that overlapped with human and chimpanzee segmental duplication [36]. These are genomic duplications characterized by >1 kb and >90% identity. Four out of 90 numts showed overlap with segmental duplications. In the cases where numts overlap duplicated segments, numts were found in only one of the copies while missing from the others, which demonstrated that numts were inserted subsequent to the duplication events. Therefore, recent numts described in this study cannot be explained by non-allelic homologous recombination.

A numt duplication mechanism that is independent of homology was the next possibility that was considered. Promiscuous DNA template switching is the only copying mechanism that was reported to cause genome structural variations in a homology-independent manner [37][39]. According to this mechanism, a stalled replication fork invades a nearby template at another DNA replication fork and copies the information available at that locus. The meaning of this mechanism while considering numt insertion is that the fork should switch to an alternative template that includes a preexisting numt, copy the numt and switch back to the original template in a position that is continuous to the first one. This is very unlikely. Nevertheless, the possibility that species-specific numts may arise by this mechanism was tested. If numts emerge by promiscuous DNA template switching, one should see additional numt copies in the genome of at least the same size, which can act as templates. In addition, if a numt has arisen by template switching, it should be phylogenetically more closely related to the donor numt than to the mitochondria. Based on DNA distance analysis (see Methods) only 18 out of 90 numts showed lower DNA distances to another nuclear sequence than to the mitochondrial one. This number is an upper estimation for numts that may have potentially arisen through homologous-independent duplication, since independently arising numts can be closely related to one another if they arose in the temporal proximity [20]. Therefore, these 18 numts were analyzed further. DNA template switching occurs in cis, namely within the same chromosome [38][40], which is due to the need to invade an adjacent replication fork [40] and in primates due to chromosome territory [41]. Out of the 18 suspected numts, only one could have potentially arisen from a preexisting numt on the same chromosome. In addition, none of the numts has additional DNA fragments from a donor site, as is expected from template switching mechanism. Finally, promiscuous DNA template switching involves considerable rearrangements of the chromosome, usually with more than a single break point and covers long genomic regions (0.2–7 Mb [38]). Numt insertions, on the other hand, are short (0.03–6 Kb) and show a simple and local insertion pattern. Taken together, in our study we conclude that the most parsimonious explanation for species-specific numt integration is NHEJ.

Human and chimpanzee numt loci can be used to deduce the mechanism of NHEJ. The hominoid lacking a particular numt carries the sequence prior to DNA repair, while the hominoid carrying that numt has the sequence reflecting the DSBR event. Thus, the differences between the chromosomal numt loci sequences of these two hominoids can be considered to reflect directly NHEJ processing (Figure S1 and Figure 2). Similar argument was previously used for analysis of few human polymorphic numts where individuals differ in the presence of a numt in specific loci [16]. Note that this inference is possible because human and chimpanzee share a recent common ancestor (Figure 1) with a low mean single-nucleotide substitution rate between their genomes of 1.23% [42].


Figure 2. Common forms of numt-mediated NHEJ.

Each alignment includes a human or chimpanzee locus with a numt as well as the corresponding nuclear sequence in the sister taxon and rhesus sequences. The mitochondrial sequences from human and chimpanzee are also indicated. (A) Numt insertion involved microhomology of AGC at the left fusion point (shown in red) and blunt-end repair at the right fusion point [id = 97]. (B) The numt locus involved imperfect microhomology at the left fusion point and continuous microhomology at the right fusion point. The position marked with an arrow has C in the nuclear genomes but G in the mitochondrial genomes. One base was deleted (green) [id = 16]. (C) Numt insertion involved a blunt-end repair with non-templated insertions of two bases at the left fusion point and three bases at the right fusion point (shown in yellow) [id = 19]. (D) Numt insertion involved microhomology of two bases on one side (shown in red on left, but could also be on the right) and a chromosomal target sequence duplication of ten nucleotides (blue) on the other side [id = 32]. (E) Numt insertion involved microhomology-dependent repair at both fusion points (red). Nineteen bases that appeared in the corresponding locus in the human and rhesus monkey genomes were deleted from one or both of the fusion points [id = 74]. (F) Numt insertion was mediated by a blunt-end repair event. One base was deleted (green) [id = 44]. The numt region is shown in a gray box. Deletions (in green) are shown in the gray box. Numts are trimmed and their size is indicated.


At each end of a numt, there is a junction with chromosomal DNA to one side and mitochondrial DNA on the other, and these junctions reflect the repair events at each end of the original chromosomal break (left or right in Figure 2). Repair of the chromosomal sides of the fusion point can be studied by examining the sequences at the junction, but the donor mitochondrial DNA used to patch the chromosomal DSB cannot be studied since it is no longer present.

To assess repair at the numt integration sites, numts were examined for deletions in the flanking chromosomal DNA and for evidence of microhomology- versus blunt-directed repair. All 90 species-specific numts were tested for evidence of deletions. Of 180 fusion points (90×2 fusion points/numt), 152 were analyzed further for microhomology and blunt-end repair (Table 1). Twenty-eight fusion points were excluded from the microhomology and blunt-end repair analysis due to uncertainty in classification (see below and Methods). All numts as well as their classifications are shown in Table S1.


Table 1. 90 human and chimpanzee numts appear in this study and their classification to numt-chromosome fusion point. Numts are shown according to their two-side classification.


Frequency of Repair Involving Microhomologies Supports Cassical NHEJ during numt Integrations

Microhomology in chromosomal NHEJ has been observed in numerous cases [2],[43],[44]. Some reports have shown that repair that involves microhomology is a statistically significant mechanism of NHEJ, but a single base microhomology is not [12],[45]. While classical NHEJ is very effective even in the absence of microhomology [3],[44], alternative end joining pathways rely almost exclusively on microhomology-related repair [43],[46]. A dataset of 152 junctions was used to study the role of repair involving microhomologies in numt integration.

End-joining involving use of microhomology is inferred at the fusion point when both human and chimpanzee chromosomal nuclear sequences overlap with the mitochondrial sequence (e.g., Figure 2A, left side; Figure S2). Eighty-four fusion points involved microhomology of 1–7 bp, in agreement with experimental data [2],[44],[45].

The null hypothesis that microhomology is not involved in numt insertion, but appears by chance at the point of integration between nuclear and mitochondrial DNAs was tested. This was done by examining 15,200 fusion points of random computer-generated blunt end breaks within the human and mitochondrial genomes (Figure 3). The null hypothesis was rejected (one-tail Fisher exact test, P = 1.3×10−12), thus, microhomology is involved in the insertion of numts. A single base is frequently considered sufficient length for repair mediated by microhomology [6],[47],[48]. According to our analysis, microhomology of a single base was not statistically different from a random single base microhomology (binomial test, n = 3138, p = 1/101, k = 40, P = 0.064).


Figure 3. Statistical analysis of DSBR involving microhomologies.

The percentages of numts (grey) and random (white) datasets with a specific microhomology length are shown. The numt dataset includes 152 genome-numt fusion points, and the random dataset includes 15,200 randomly chosen genome and mitochondrial positions. A one-tail Fisher exact test (presence/absence of microhomology, P = 1.3×10−12) was statistically significant. A star indicates a significant difference between numt and random datasets for a given microhomology length (binomial test, P<0.05/6). The percentage of imperfect microhomology is shown at the top of the graph with a diagonal pattern. Imperfect microhomology was higher in the numt dataset than in the random dataset (Fisher exact test, all P = 0.01, microhomology of 1, P = 0.0023).


Imperfect microhomology, i.e., microhomology with a mismatch, was considered (Figure 2B). One mismatch was allowed per microhomology stretch, and the base that is adjacent to the fusion point must show microhomology. Only cases where a mismatch in the microhomology stretch increased the stretch by at least two bases were counted. We found that imperfect microhomology is more common in the real numt dataset then in the random one (one-tail Fisher exact test, P = 0.01). This was highlighted in the case of single microhomologies (one-tail Fisher exact test, P = 0.0024). Therefore, repair events involving a single base microhomology were treated as blunt-end repair events, except for those cases that show imperfect microhomology, which were treated as microhomology events. Using this revised definition, 53 (35%) cases of numt-genome fusion points employed significant microhomology in the DSBR event (Table 1, see Table S2 for consideration of single base microhomology as microhomology). Thus, it appears that repair involving microhomology plays some role in numt integration but is not totally required, as is the case in classical NHEJ. Thus, our extensive dataset provides evidence that repair involving microhomology during NHEJ that was experimentally focused on specific break configuration and genomic locations is a genome-wide phenomenon.

Target Sequence Duplication during Blunt-End Repair Reveals the Structure of Native Breaks

Ninty-nine repair events (65%) either did not involve any microhomology, or involved only one base microhomology and were considered to be blunt-end repair events (Table 1; Figure 2A right; Figure S2). The processing of numt-chromosome blunt fusion points was analyzed. The focus of this section is events that were processed by nucleotide insertions. Nucleotide deletions will be discussed in the next section. In 26 of the 99 blunt-end repair events, nucleotide additions were identified in addition to the numt insertion. Nine of these involved insertion of nucleotides that could not be explained by synthesis using a DNA template (Figure 2C). DNA polymerases that act in DNA template-independent manner tend to insert only few nucleotides [49][51]. Therefore, we arbitrarily limited the insertion repair events to addition of ≤5 nucleotides (Table 1). Ten larger insertion events were removed from fusion point classification as the numt integration likely followed another DNA capture event (Figure S1; Table 1).

In 17 of 71 numt integration events (24%) a second type of nucleotide insertion during numt integration was observed, where duplication of short chromosomal sequences flanking numt fragment was detected (Figure 2D right side). Duplication length ranged from one to 11 nucleotides. These target sequence duplications can occur when 5′ or 3′ cohesive breaks are separated by the mitochondrial DNA following single stranded DNA gap filling during the repair. While the mechanism of integration is different, duplication of the target sequence has been shown for transposable elements such as LINEs [52]. Since numts do not induce breaks, but are passively captured into preexisting breaks, we argue that target duplicated sequences represent overhang ends of naturally occurring DSBs. To the best of our knowledge, this is the most comprehensive description of the configuration of naturally occurring DSBs.

Two important insights are drawn from this analysis. First, 24% of integration events were associated with cohesive ends. We suggest that cohesive breaks are probably more frequent in the genome than observed here since those breaks need no processing and are likely to be repaired accurately without patching by numts. Second, numts inserted in breaks with up to 11 nucleotide overhangs were detected, suggesting that staggered SSBs spaced by 11 nucleotides can lead to a DSB. Since DSBs are more dangerous to genome stability than SSBs it is important to know how close two SSBs on opposite strands should be to be considered a DSB [53].

Numt Insertions Involve Reduced Loss of Chromosomal Nucleotides

The fusion of mitochondrial and chromosomal DNA likely occurs between incompatible ends, i.e., non-annealing overhangs. In NHEJ studies where filler DNA is not involved, DSBR of incompatible ends always involves deletion of a few nucleotides [3],[5],[54]. For example, in 96 events of Vκ-Jκ recombination, the most studied system for incompatible ends, only one event did not involve deletions [3],[54]. Similarly, in a study of incompatible I-SceI cleavage repair, none of 59 clones preserved the 4-nucleuotide overhangs from the sides of the break [5]. Surprisingly, of the 90 numt insertions (Table 1) only 41 (46%) involved deletions of nucleotides from the chromosome (e.g., Figure 2B, E, F). The other 49 insertions (54%) did not involve deletions of even a single nucleotide (Figure 4A). Unlike endonuclease generated breaks, random breaks often involve chemical degradation of the bases at the breakpoint prior to repair [53]. Out of 41 numt insertion events that involved deletions, 12 were deletions of a single base, which could have occurred prior to repair. Thus, the frequency of deletions caused by the integration of numts could be lower than the observed deletion frequency.


Figure 4. Numt insertions involve reduced loss of chromosomal nucleotides.

Pie diagrams show the distribution of deletion sizes in our study and different experimental systems with filler DNA (A) numt-mediated repair usually involves either no deletions or small 1–2 base deletions, (B) Spontaneous integration of AAV [56][58] (C) Random integration into I-SceI [12],[13],[55], and (D) Integration of AAV into I-SceI [56] include more deletions. One-tail Fisher exact tests (presence/absence of deletions, numt versus spontaneous AAV P = 9.5×10−6, numt versus AAV P = 4.7×10−6, and numt versus random P = 0.001) were statistically significant. Similarly, the meanrank deletion size in the numt group is significantly smaller than in the other groups (Kruskal–Wallis nonparametric ANOVA n = 183, P = 10−13; A posteriori Tukey-Kramer test P = 0.01). Scale is 0–9 bases, and deletions bigger than 9. Offset slice shows no deletions. A detailed list of deletion size for all non-numt events appears in Table S3.


In numt-mediated NHEJ mitochondrial DNA acts as filler DNA that may provide the repair machinery an alternative to nuclease activity during chromosome processing. Therefore, this evolutionary study was compared with studies where filler DNA was captured into mammalian chromosomes experimentally [12], [13], [55][58]. The filler DNA types that were included in this comparison were spontaneous integration of Adeno-Associated Virus (AAV) into chromosomes, integration of AAV into I-SceI induced breaks and integration of other types of filler DNA into I-SceI sites ([12], [13], [55][58], Figure 4). We tested the null hypothesis that the proportion of cases that did not involve deletions would be similar in the numt and the experimental filler DNA systems. Spontaneous integration of AAV into genomes involved deletions in the vast majority of cases (24/26, 92% [56][58], see Figure 4B). A similar picture was obtained from integration of AAV into I-SceI sites, with 22/23 events (96%) involving deletions ([56], Figure 4D). This effect is not limited to AAV. When other types of DNA [12],[13],[55] (e.g., pCMV and φX174) are captured to I-SceI induced breaks 33/44 events (75%) involve deletions ([12],[13],[55], Figure 4C). We found that the null hypothesis can be rejected and that numt-mediated NHEJ involves more events without deletions than experimental systems regardless of the filler DNA used (one-tail Fisher exact tests: numt verses spontaneous AAV P = 9.5×10−6, numt verses AAV P = 4.7×10−6, and numt versus random P = 0.001).

Moreover, numts show a higher frequency of very small deletions (1–2 bases) in comparison to the other three filler DNA studies and a lower frequency of larger deletions (Figure 4). In a comparison of the overall deletion size between repairs involving numts versus other filler DNA, the deletion size in numt repair events was significantly smaller than the deletion size in all of the other filler DNA groups (Kruskal–Wallis nonparametric ANOVA, n = 183, P = 10−13, mean ranks of 65.5, 142.5, 99.3 and 128.7 for numts, spontaneous AAV, random filler DNA, and I-SceI AAV reciprocally. A posteriori Tukey-Kramer test P = 0.01). In conclusion, numts show less frequent and smaller deletions in comparison to the other three types of filler DNA.

All numts described here are inserted into non-coding DNA. Both deletions and insertions are very common during primate evolution [59],[60]. Hence, there is no reason to assume that numt insertion events that do not involve deletions are favored over ones that do involve deletions of few bases. Therefore, the low frequency of chromosomal deletions during numt insertion is likely to stem from a mechanistic feature of insertion rather then post-insertion selection.

Mechanism of Deletion Prevention Performed by numts

To understand the mechanisms responsible for reduced chromosomal deletion during numt integration we went back to our DSBR analysis (Table 1). In 71 cases where we could analyze the two sides of the integration events (Table 1), 30 showed no microhomology, 33 showed microhomology only in one side, and only 8 showed microhomology on both sides. This suggests that the two sides of DSBs repaired by numt insertion are seen independently by repair machinery at least in regard to microhomology- and blunt-end-directed repair.

Interestingly, numt insertion via blunt-end repair on both sides of the insertion resulted in the lowest proportion of deletions (7/30). Events involving numt insertion via blunt-end repair on one side and microhomology on the other also resulted in a low proportion of deletions (14/33). In sharp contrast, events where both sides of the numt were patched through repair involving microhomologies almost always involved deletions (7/8). We conclude that the low deletion rate is not due to selection of numts that mimic the complementary strand of chromosomal overhangs, but rather due to the ability to perform blunt-end ligation without trimming chromosomal ends.


Primate genomes are useful for studying the repair of naturally occurring DSBs via numt integration because of their high sequence similarity and the availability of multiple complete genomes. While our analysis identified similarities between the pattern of numt integration in primate evolution and experimental NHEJ repair patterns (see below), it also highlighted a clear difference between them.

In repair events of incompatible ends, small deletions always occur [3],[5],[6]. Deletions during repair are also common in repair involving filler DNA (Figure 4). While only reports which include raw data are shown in Figure 4, recently Miller et al. reported that about 80% of 212 random integration events of AAV into human genome involved deletions larger than 10 bases, and about 30% of the 212 events involving deletions larger than 100 bases from the host chromosome [61].

In contrast, only 46% of numt integration events included deletions, and those deletions were small. The differences between the frequency and size of deletion events in numt-mediated DSBR and other filler DNA mediated DSBR are statistically significant. Since selection is likely to act similarly on loci with no deletions or only a few base deletions in non-coding regions, the fact that so many numt integration events do not involve deletions at all, suggests it is determined by the mechanism of insertion and not by post-insertion selection. Our analysis indicates that numts prevent chromosomal deletions primarily through blunt-end repair. At least two mechanisms may explain how numts prevent chromosomal deletions. It could be that trimming of DNA ends occurs specifically in the mitochondrial DNA during DSBR (Figure 5A). Alternatively, or in addition, some breaks may contain degraded 3′OH on both sides such that direct ligation or ligation facilitated by DNA synthesis cannot occur. In this situation, the only way to perform end joining is by deleting bases. Numts can supply intact 3′ OH for ligation with no need for nuclease processing (Figure 5B).


Figure 5. Sealing DSBs with numts reduces the risk of deleterious DSBR–a model for protective filler DNAs.

DSBs can vary in their chemical complexity, simple breaks are breaks in which the phosphoester bond is broken but neither the sugar nor the nucleotides in the vicinity of the breaks are damaged. In contrast, complex breaks include fragmented sugar and damaged or missing bases in the vicinity of the break. While simple DSBs like experimentally induced ones are repaired with minimal processing, repair of complex DSBs can sometimes be deleterious. We propose that numts can seal complex DSBs and thus reduce the risk for deleterious repair. At least two mechanisms may explain how numts, and probably other filler DNA, prevent chromosomal deletions. (A) Numts prevent deletions if they undergo selective processing that enables them to seal the broken chromosome. For example, if exposure of long single-strand DNA occurs only in the numt sequence, numt-genome repair mediated by microhomology (dots) can be accomplished even if very short chromosomal single-stranded DNA is exposed at the immediate vicinity of the break. Thus, only non-matching mitochondrial DNA will be removed. (B) Alternatively, some breaks may contain degraded 3′OH in both sides. Numts can supply the intact 3′ OH to perform ligation with no need for nuclease processing. (C) In the absence of filler DNA (i.e., numts), repair of complex DSBs may not occur in the immediate vicinity of DSB, but instead single-stranded DNA is exposed followed by a search for microhomology between the two sides of the breaks (marked here in strips). When microhomology is found, the two sides of the breaks are annealed, and non-complementary single-strand DNA is trimmed off (scissors) to yield a deletion in the chromosome. Dotted lines represent DNA synthesis and crescent-shaped blobs represent endonucleases.


Although numt integration events show a lower frequency of deletion than V(D)J recombination their repair patterns are similar. The frequency of repair involving microhomology is similar in both cases as well as the limited processing of the chromosomal DNA. Therefore, it is reasonable to suggest that numts are inserted using classical Ku-dependent, DNA ligase IV-dependent NHEJ [5],[43],[62], and that the information from experimental systems holds true for broader genetic loci and end configurations.

In a broader context numts can serve as a model for other types of filler DNA which are frequently found both in evolution and in experimental DSBR. We propose a model in which some types of filler DNA may have a role in protecting chromosomes from deleterious deletions during DSBR (Figure 5). DSBs can vary in their chemical complexity. Simple breaks are breaks in which the phosphoester bond is broken but neither the sugar nor the nucleotides in the vicinity of the breaks are damaged [63]. In contrast, complex breaks include fragmented sugar damaged or missing bases in the vicinity of the break [64],[65]. While simple DSBs generated by endonucleases mainly cause small deletions, it is clear that a subset of random breaks leads to large deletions and translocations [43], [66][68]. For example, ionizing radiation induced hprt mutants resulted in deletions up to 56 kb whereas improper IgH class switching and spontaneous DSBs can lead to translocations [43], [66][68]. Molecular characterization of large deletions and translocations revealed that they are mainly caused by microhomology-mediated repair [8],[43],[67],[69]. Genetic analysis showed that these types of deletions and translocations are probably formed by alternative end-joining [5],[43],[62], where DSBs are processed by nucleases to generate long stretches of single stranded DNA, followed by a microhomology search. When microhomology is found, the two sides of the breaks are annealed, and non-matched single-strand DNA is trimmed off to yield a deletion (Figure 5C). Our results are consistent with a model in which filler DNA may seal breaks and prevent their processing into long single stranded DNA (Figure 5A, B).

Deletions are reduced during NHEJ by restricting the activity of nucleases primarily by the Ku heterodimer [5]. Nuclease activity is limited further during V(D)J recombination by Rag1/2. Deletions also are reduced during V(D)J recombination by DNA polymerase μ [54],[62]. Our results suggest that filler DNA such as numt can act similarly to reduce deletion size during the repair of random breaks. Numts might carry this out by increasing the efficiency of repair processes that do not involve nuclease activity, for example, by providing an intact 3′OH for DNA polymerases or DNA ligase.

Capture of DNA sequences into chromosomal breaks was shown previously both in cell culture and during chromosomal evolution [12], [13], [55][58],[70]. It also was suggested that the captured DNA serves as a repair factor [70]. However, capture of DNA has not been shown to protect the chromosome from deletions, as is indicated here. In fact, as shown in Figure 4 in some experimental designs, capture of DNA into breaks involved frequent and substantial deletions of the chromosome. There are several possible explanations as to why the results obtained with numts are different from the results that obtained experimentally (Figure 4).

First, the specific experimental design may influence the repair outcomes. Numts are captured into variety of break configurations while DNAs in Figure 4C and D are captured into defined I-SceI breaks. It was shown that incompatible 3′-overhang-breaks that are present during the repair of I-SceI sites with filler DNA are prone to deletions [44]. In addition, during the experiment, the genome undergoes cycles of I-SceI cleavage and end joining until the filler DNA disrupts the I-SceI recognition site. During these cycles, some repair factors can be exhausted that may affect the repair outcomes. Alternatively, the repeated formation of DSBs can signal the cell as if an un-repairable DSB is formed that may lead to a change in the cellular repair strategy [6].

Second, different cell types and developmental stages sometimes can show different repair outcomes [71],[72]. While numts in our study are inserted into the germlines in vivo, the experiments that are presented in Figure 4 B–D were performed in immortalized cells in culture.

Finally, a very important factor that may explain the differences between numt-mediated repair and other filler DNAs is the type of DNA that is captured into the break. It appears that some filler DNA can “protect” from DNA deletions during insertion, while other filler DNA may be promiscuous, promoting alterations. Capture of AAV into both random and I-SceI breaks showed similar results with the highest frequency of deletions (Fig. 4B,D), indicating that in this case the type of DNA has more effect than the type of break. AAV DNA is a single stranded DNA that forms a loop structure at one side and a long single strand tail at the other. Integration sites of AAV to the genome occur in the loop structure [56], therefore the loop structure should be opened up and processed before ligation. During this process, a unique DNA structure of non-complementary base-pairs is formed at the end of the AAV DNA. This structure probably makes blunt end ligation with the chromosome very unlikely to occur. Here, we showed that numt integrations prevent deletions mainly through blunt-end repair. It is possible that capture of DNA that cannot be blunt-ligated occurs via a different mechanism that is prone to form deletions. Indeed, the mechanism of insertion of AAV into genomes as suggested by Miller et al. [56] is opposite to what we describe here for numts. According to their model, AAV integration is promoted by exposure of long ssDNA followed by deletions.

Unlike AAV, the capture of different types of DNA into I-SceI breaks (figure 4C) was more similar to the capture of numts with respect to deletion frequency. In addition to the explanations discussed above, in figure 4C different types of sequences are captured into the breaks including capturing of retrotransposon cDNA [12],[13]. Recently, it was shown that retrotransposon cDNA can be captured passively into breaks both in cell cultures and during primate evolution via an NHEJ-independent pathway. This process involves a high frequency of associated deletions (86%) and a large deletion size (up to 14 kb) [70],[73]. Therefore, it can be that the differences that are observed between panels A and C (Figure 4) could be explained partially by the capture of cDNA or other DNA that is integrated similarly.

We speculate that within cells there are others DNA fragments that are captured into DSBs with a similar protective potential as numts. Further study of the insertion mechanism of other passively captured filler DNA will identify protective fragments and potentially the rules in their preferential use over promiscuous filler DNA during chromosome evolution. This will add valuable insights into the mechanisms of chromosomal evolution and maintenance of genome integrity.


Identification of Human and Chimpanzee Species-Specific numts

Genomic sequences and annotations were obtained from the University of California at Santa Cruz [74] Genome Center. The genome versions used were hg18 (human, Homo sapiens), panTro2 (chimpanzee, Pan troglodytes), and RheMac2 (rhesus monkey, Macaca mulatta). The pair-wise analysis is described in Hazkani-Covo and Graur (2007). In short, BLAST was used to search each of the human and chimpanzee genomes for regions of similarity with mitochondrial sequences. Numts were then classified as new based on the alignment files: netPanTro2 for human-chimpanzee, netHg18 for chimpanzee-human, and two netRheMac2 tables for human-rhesus and chimpanzee-rhesus. Numts that appeared in one of the two hominoids (human or chimpanzee) and gaps that appeared in the reciprocal locations in the two other genomes were identified. A total of 90 numts were identified based on this criteria and are discussed in this study. Numts that appeared in one of the two hominoids [18] but could not be compared to the rhesus genome for that loci, were not used in this study.

DSBR Analysis

First, the possibility that numt insertion occurred via homologous recombination between chromosomal and mitochondrial DNA or between chromosomal DNA and nuclear paralogous numt was tested. The test was based on the assumption that if such recombination had occurred a significant portion of both the numt and its flanking regions should be homologous to a region of the nuclear or mitochondrial genome. To test those options, we BLASTed numt including 100 bases of flanking genomic sequence from each side of the fusion point against the nuclear and mitochondrial genomes looking for homologies that cover both the numts and the flanking regions. We looked for hits that covered more than 10 bp of each of the flanking region [75].

In order to test overlap of numts with segmental duplication, the recent human (Hg18) and chimpanzee (PanTro2) segmental duplications were downloaded from the segmental duplication database [36]. Segmental duplications of fragments that include numt regions were tested against the mitochondria using BLAST2SEQUENCES.

To test the option of homologous-independent numt duplication, Hasegawa-Kishino-Yano (HKY) DNA distances were calculated using PAUP [76]. Distances were calculated between each numt and its mitochondrial fragment and between each numt and a possible nuclear template. Numts that show lower DNA distances to a nuclear copy rather than to the mitochondria were analyzed.

Loci of species-specific numts were aligned with the two other nuclear genomes and with mitochondria from human and chimpanzee. Flanking genomic regions of 100 bp were used from each side of the numt. Alignments were created with ClustalW [77] and inspected manually.

Numts then were classified for the underling NHEJ patterns (microhomology, blunt end repair, and deletion size). Each side of the numt was analyzed independently according to the following parameters. First microhomology was indicated only if the nucleotide adjacent to the fusion point was shared between the numt, the comparative hominoid genome, and the mitochondrion of human or chimpanzee. The length of microhomology was defined as a set of continuous sites that each obeyed this rule. Available numt polymorphic sites were used in the analysis. If no microhomology was observed, then the DSBR was classified as a blunt-end repair event. Insertions were classified as a chromosomal target sequence duplication if the insertion was identical to the nucleotide(s) appearing in a genomic sequence on the other side of the numt. All other insertions were classified as non-template insertions. Where insertion of more than five nucleotides in a non-templated manner was observed in addition to the numt insertion, the fusion-point was removed from the study (Table 1). Genomic deletion events were counted per numt rather than per fusion point. Deletions were detected based on comparisons between genomes with and without a numt at a particular location. While numts with poorly aligned flanking regions were not included in the fusion point classification due to uncertainty (Table 1), estimates of the size of deletion was reported. Overestimation of deletion was determined in case of doubt.

Supporting Information

Figure S1.

Chimpanzee genome is used to identify numt-genome fusion points of human specific numts. Five human genomic loci with numt polymorphisms are shown. Each alignment includes two human variants (with and without the numt) as well as the corresponding chimpanzee and rhesus sequences. The numt region is shown in a gray box, and the conservation line between human and chimpanzee is presented. (A–C) Cases where the sequences around numt integration site are identical between the numt polymorphic (NP) human variant and that of chimpanzee. (D) An independent tandem duplication of CAAA occurred in the lineage leading to human. (E) The comparative genome approach is not always useful. At the right side of the numt-genome fusion point, an insertion of fourteen bases is observed in addition to the numt insertion. The event that occurred at the left numt-genome fusion point is not clear since the AGG triplet can be aligned on both sides of the gap that was formed in the alignment. Cases like E were not included in the fusion point analysis. The following refSNP ID and study case numbers were used: rs35075891, id = 1; rs35867387, id = 3; rs1052523, id = 22; rs11271404, id = 6; and rs34351771, id = 31.


(3.54 MB EPS)

Figure S2.

Repair of a DSB by numt-mediated NHEJ using microhomology and blunt-end repair sub-mechanisms. (A) A non-cohesive putative DSB is created in the chromosome strands with a blunt-end on the left side and a protruding end of 2 bases on the right side. The DSB is then repaired by a putative mitochondrial piece (gray) yielding a blunt-end repair reaction on the left and a microhomology-mediated reaction with two bases on the right. (B) The alignment of the repaired DNA in A (upper sequence) with the corresponding loci in two closely related species is shown. Note that on the right fusion-point where microhomology-mediated repair happened an overlap of two bases appears between the two intact sequences and the mitochondrial sequence (grey).


(0.47 MB EPS)

Table S1.

Detailed classification of numts that are analyzed in this study. For each of the 90 numts, information about chromosome and mitochondria coordinates as well as the DSBR pattern for left and right fusion points are indicated. Blunt-end repair/microhomology-mediated repair as well as non-template insertions are indicated for left and right separately. In case of microhomology the actual bases are given. In addition, scores of microhomology for 8 bases upstream of the left fusion point (and downstream of the right one) are shown in a binary way, where 1 indicates a match and 0 a mismatch. For example, the alignment of CAG appear in Figure 3d (id = 74) is 00100111 (The binary code is decoded from right to left as match-match-match (CAG) mismatch-mismatch-match-mismatch-mismatc​h).Note that octamer orientation is the same as the sequence orientation and is opposite for left and for right fusion points. Cases where non-continuous microhomology was determinate are indicated in the last column (e.g., id = 99, L-3p2 means in the left side 3 bases of microhomology can be extended by two additional bases when allowing a mismatch). Template insertions as well as deletions are indicated per numt rather than per fusion point. aNumt includes additional insertion of at least 5 bases. This insertion is, therefore, suspected to be a separate event. Only the side without insertion was counted for the DSBR patterns. b Classification of numt to its repair pattern was ambiguous, therefore only deletion size was estimated.


(0.06 MB XLS)

Table S2.

90 human and chimpanzee numts appear in this study and their classification to numt-chromosome fusion point. Microhomology of a single base is classified here as microhomology in contrast to Table 1 where a single base microhomology is considered as blunt-end repair. Numts are shown according to their two-side classification. For a detailed description of each fusion point, see Table S1.


(0.05 MB DOC)

Table S3.

Deletion size in cases of NHEJ with filler DNA. For deletion size of numt-mediated repair see Table S1.


(0.04 MB DOC)


We thank J. Sekelsky, S.P. Otto, M.A. Resnick, L.J. Rasmussen, W.C. Copeland, J.M. Mason, T.J. Vision, D. Graur, and T. Dagan for discussions.

Author Contributions

Conceived and designed the experiments: EHC SC. Performed the experiments: EHC. Analyzed the data: EHC SC. Wrote the paper: EHC SC.


  1. 1. Bassing CH, Alt FW (2004) The cellular response to general and programmed DNA double strand breaks. DNA Repair (Amst) 3: 781–796.
  2. 2. Lieber MR, Ma Y, Pannicke U, Schwarz K (2004) The mechanism of vertebrate nonhomologous DNA end joining and its role in V(D)J recombination. DNA Repair (Amst) 3: 817–826.
  3. 3. Ramsden DA, Paige CJ, Wu GE (1994) Kappa light chain rearrangement in mouse fetal liver. J Immunol 153: 1150–1160.
  4. 4. Capp JP, Boudsocq F, Besnard AG, Lopez BS, Cazaux C, et al. (2007) Involvement of DNA polymerase mu in the repair of a specific subset of DNA double-strand breaks in mammalian cells. Nucleic Acids Res 35: 3551–3560.
  5. 5. Guirouilh-Barbat J, Huck S, Bertrand P, Pirzio L, Desmaze C, et al. (2004) Impact of the KU80 pathway on NHEJ-induced genome rearrangements in mammalian cells. Mol Cell 14: 611–623.
  6. 6. Honma M, Sakuraba M, Koizumi T, Takashima Y, Sakamoto H, et al. (2007) Non-homologous end-joining for repairing I-SceI-induced DNA double strand breaks in human cells. DNA Repair (Amst) 6: 781–788.
  7. 7. Rebuzzini P, Khoriauli L, Azzalin CM, Magnani E, Mondello C, et al. (2005) New mammalian cellular systems to study mutations introduced at the break site by non-homologous end-joining. DNA Repair (Amst) 4: 546–555.
  8. 8. Singleton BK, Griffin CS, Thacker J (2002) Clustered DNA damage leads to complex genetic changes in irradiated human cells. Cancer Res 62: 6263–6269.
  9. 9. Ward JF (2000) Complexity of damage produced by ionizing radiation. Cold Spring Harb Symp Quant Biol 65: 377–382.
  10. 10. Han K, Lee J, Meyer TJ, Wang J, Sen SK, et al. (2007) Alu recombination-mediated structural deletions in the chimpanzee genome. PLoS Genet 3: 1939–1949.
  11. 11. Ahnesorg P, Smith P, Jackson SP (2006) XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining. Cell 124: 301–313.
  12. 12. Lin Y, Waldman AS (2001) Promiscuous patching of broken chromosomes in mammalian cells with extrachromosomal DNA. Nucleic Acids Res 29: 3975–3981.
  13. 13. Lin Y, Waldman AS (2001) Capture of DNA sequences at double-strand breaks in mammalian chromosomes. Genetics 158: 1665–1674.
  14. 14. Moore JK, Haber JE (1996) Capture of retrotransposon DNA at the sites of chromosomal double-strand breaks. Nature 383: 644–646.
  15. 15. Ricchetti M, Fairhead C, Dujon B (1999) Mitochondrial DNA repairs double-strand breaks in yeast chromosomes. Nature 402: 96–100.
  16. 16. Ricchetti M, Tekaia F, Dujon B (2004) Continued colonization of the human genome by mitochondrial DNA. PLoS Biol 2: E273.
  17. 17. Yu X, Gabriel A (1999) Patching broken chromosomes with extranuclear cellular DNA. Mol Cell 4: 873–881.
  18. 18. Hazkani-Covo E, Graur D (2007) A comparative analysis of numt evolution in human and chimpanzee. Mol Biol Evol 24: 13–18.
  19. 19. Lopez JV, Yuhki N, Masuda R, Modi W, O'Brien SJ (1994) Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat. J Mol Evol 39: 174–190.
  20. 20. Bensasson D, Feldman MW, Petrov DA (2003) Rates of DNA duplication and mitochondrial DNA insertion in the human genome. J Mol Evol 57: 343–354.
  21. 21. Hazkani-Covo E, Sorek R, Graur D (2003) Evolutionary dynamics of large numts in the human genome: rarity of independent insertions and abundance of post-insertion duplications. J Mol Evol 56: 169–174.
  22. 22. Tourmen Y, Baris O, Dessen P, Jacques C, Malthiery Y, et al. (2002) Structure and chromosomal distribution of human mitochondrial pseudogenes. Genomics 80: 71–77.
  23. 23. Bensasson D, Zhang D, Hartl DL, Hewitt GM (2001) Mitochondrial pseudogenes: evolution's misplaced witnesses. Trends Ecol Evol 16: 314–321.
  24. 24. Richly E, Leister D (2004) NUMTs in sequenced eukaryotic genomes. Mol Biol Evol 21: 1081–1084.
  25. 25. Woischnik M, Moraes CT (2002) Pattern of organization of human mitochondrial pseudogenes in the nuclear genome. Genome Res 12: 885–893.
  26. 26. Mishmar D, Ruiz-Pesini E, Brandon M, Wallace DC (2004) Mitochondrial DNA-like sequences in the nucleus (NUMTs): insights into our African origins and the mechanism of foreign DNA integration. Hum Mutat 23: 125–133.
  27. 27. Goldin E, Stahl S, Cooney AM, Kaneski CR, Gupta S, et al. (2004) Transfer of a mitochondrial DNA fragment to MCOLN1 causes an inherited case of mucolipidosis IV. Hum Mutat 24: 460–465.
  28. 28. Turner C, Killoran C, Thomas NS, Rosenberg M, Chuzhanova NA, et al. (2003) Human genetic disease caused by de novo mitochondrial-nuclear DNA transfer. Hum Genet 112: 303–309.
  29. 29. Timmis JN, Ayliffe MA, Huang CY, Martin W (2004) Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes. Nat Rev Genet 5: 123–135.
  30. 30. Graur D, Shuali Y, Li WH (1989) Deletions in processed pseudogenes accumulate faster in rodents than in humans. J Mol Evol 28: 279–285.
  31. 31. Noutsos C, Kleine T, Armbruster U, DalCorso G, Leister D (2007) Nuclear insertions of organellar DNA can create novel patches of functional exon sequences. Trends Genet 23: 597–601.
  32. 32. Adams KL, Qiu YL, Stoutemyer M, Palmer JD (2002) Punctuated evolution of mitochondrial gene content: high and variable rates of mitochondrial gene loss and transfer to the nucleus during angiosperm evolution. Proc Natl Acad Sci U S A 99: 9905–9912.
  33. 33. Hedges DJ, Batzer MA (2005) From the margins of the genome: mobile elements shape primate evolution. Bioessays 27: 785–794.
  34. 34. Blanchard JL, Schmidt GW (1996) Mitochondrial DNA migration events in yeast and humans: integration by a common end-joining mechanism and alternative perspectives on nucleotide substitution patterns. Mol Biol Evol 13: 893.
  35. 35. Bailey JA, Eichler EE (2006) Primate segmental duplications: crucibles of evolution, diversity and disease. Nat Rev Genet 7: 552–564.
  36. 36. Cheng Z, Ventura M, She X, Khaitovich P, Graves T, et al. (2005) A genome-wide comparison of recent chimpanzee and human segmental duplications. Nature 437: 88–93.
  37. 37. Branzei D, Foiani M (2007) Template switching: from replication fork repair to genome rearrangements. Cell 131: 1228–1230.
  38. 38. Lee JA, Carvalho CM, Lupski JR (2007) A DNA replication mechanism for generating nonrecurrent rearrangements associated with genomic disorders. Cell 131: 1235–1247.
  39. 39. Slack A, Thornton PC, Magner DB, Rosenberg SM, Hastings PJ (2006) On the mechanism of gene amplification induced under stress in Escherichia coli. PLoS Genet 2: e48.
  40. 40. Bailey JA, Liu G, Eichler EE (2003) An Alu transposition model for the origin and expansion of human segmental duplications. Am J Hum Genet 73: 823–834.
  41. 41. Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, et al. (2007) Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol 9: 675–682.
  42. 42. CSAC (2005) Initial sequence of the chimpanzee genome and comparison with the human genome. Nature 437: 69–87.
  43. 43. Yan CT, Boboila C, Souza EK, Franco S, Hickernell TR, et al. (2007) IgH class switching and translocations use a robust non-classical end-joining pathway. Nature 449: 478–482.
  44. 44. Roth DB, Wilson JH (1986) Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction. Mol Cell Biol 6: 4295–4304.
  45. 45. Merrihew RV, Marburger K, Pennington SL, Roth DB, Wilson JH (1996) High-frequency illegitimate integration of transfected DNA at preintegrated target sites in a mammalian genome. Mol Cell Biol 16: 10–18.
  46. 46. Verkaik NS, Esveldt-van Lange RE, van Heemst D, Bruggenwirth HT, Hoeijmakers JH, et al. (2002) Different types of V(D)J recombination and end-joining defects in DNA double-strand break repair mutant mammalian cells. Eur J Immunol 32: 701–709.
  47. 47. Kabotyanski EB, Gomelsky L, Han JO, Stamato TD, Roth DB (1998) Double-strand break repair in Ku86- and XRCC4-deficient cells. Nucleic Acids Res 26: 5333–5342.
  48. 48. Willers H, Husson J, Lee LW, Hubbe P, Gazemeier F, et al. (2006) Distinct mechanisms of nonhomologous end joining in the repair of site-directed chromosomal breaks with noncomplementary and complementary ends. Radiat Res 166: 567–574.
  49. 49. Dominguez O, Ruiz JF, Lain de Lera T, Garcia-Diaz M, Gonzalez MA, et al. (2000) DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells. Embo J 19: 1731–1742.
  50. 50. Moon AF, Garcia-Diaz M, Bebenek K, Davis BJ, Zhong X, et al. (2007) Structural insight into the substrate specificity of DNA Polymerase mu. Nat Struct Mol Biol 14: 45–53.
  51. 51. Ramadan K, Maga G, Shevelev IV, Villani G, Blanco L, et al. (2003) Human DNA polymerase lambda possesses terminal deoxyribonucleotidyl transferase activity and can elongate RNA primers: implications for novel functions. J Mol Biol 328: 63–72.
  52. 52. Smit AF (1996) The origin of interspersed repeats in the human genome. Curr Opin Genet Dev 6: 743–748.
  53. 53. Caldecott KW (2007) Mammalian single-strand break repair: mechanisms and links with chromatin. DNA Repair (Amst) 6: 443–453.
  54. 54. Nick McElhinny SA, Havener JM, Garcia-Diaz M, Juarez R, Bebenek K, et al. (2005) A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining. Mol Cell 19: 357–366.
  55. 55. Liang F, Han M, Romanienko PJ, Jasin M (1998) Homology-directed repair is a major double-strand break repair pathway in mammalian cells. Proc Natl Acad Sci U S A 95: 5172–5177.
  56. 56. Miller DG, Petek LM, Russell DW (2004) Adeno-associated virus vectors integrate at chromosome breakage sites. Nat Genet 36: 767–773.
  57. 57. Miller DG, Rutledge EA, Russell DW (2002) Chromosomal effects of adeno-associated virus vector integration. Nat Genet 30: 147–148.
  58. 58. Nakai H, Montini E, Fuess S, Storm TA, Grompe M, et al. (2003) AAV serotype 2 vectors preferentially integrate into active genes in mice. Nat Genet 34: 297–302.
  59. 59. Mills RE, Luttig CT, Larkins CE, Beauchamp A, Tsui C, et al. (2006) An initial map of insertion and deletion (INDEL) variation in the human genome. Genome Res 16: 1182–1190.
  60. 60. Wetterbom A, Sevov M, Cavelier L, Bergstrom TF (2006) Comparative genomic analysis of human and chimpanzee indicates a key role for indels in primate evolution. J Mol Evol 63: 682–690.
  61. 61. Miller DG, Trobridge GD, Petek LM, Jacobs MA, Kaul R, et al. (2005) Large-scale analysis of adeno-associated virus vector integration sites in normal human cells. J Virol 79: 11434–11442.
  62. 62. Corneo B, Wendland RL, Deriano L, Cui X, Klein IA, et al. (2007) Rag mutations reveal robust alternative end joining. Nature 449: 483–486.
  63. 63. Monteilhet C, Perrin A, Thierry A, Colleaux L, Dujon B (1990) Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. Nucleic Acids Res 18: 1407–1413.
  64. 64. Inamdar KV, Pouliot JJ, Zhou T, Lees-Miller SP, Rasouli-Nia A, et al. (2002) Conversion of phosphoglycolate to phosphate termini on 3′ overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1. J Biol Chem 277: 27162–27168.
  65. 65. Sutherland BM, Bennett PV, Sidorkina O, Laval J (2000) Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation. Proc Natl Acad Sci U S A 97: 103–108.
  66. 66. O'Neill P, Nicklas J, Hirsch B, Jostes R, Hunter T, et al. (2005) In vitro studies of the genotoxicity of ionizing radiation in human G(0) T lymphocytes. Environ Mol Mutagen 46: 207–220.
  67. 67. Putnam CD, Pennaneach V, Kolodner RD (2005) Saccharomyces cerevisiae as a model system to define the chromosomal instability phenotype. Mol Cell Biol 25: 7226–7238.
  68. 68. Sankaranarayanan K (1991) Ionizing radiation and genetic risks. III. Nature of spontaneous and radiation-induced mutations in mammalian in vitro systems and mechanisms of induction of mutations by radiation. Mutat Res 258: 75–97.
  69. 69. Monnat RJ Jr, Hackmann AF, Chiaverotti TA (1992) Nucleotide sequence analysis of human hypoxanthine phosphoribosyltransferase (HPRT) gene deletions. Genomics 13: 777–787.
  70. 70. Sen SK, Huang CT, Han K, Batzer MA (2007) Endonuclease-independent insertion provides an alternative pathway for L1 retrotransposition in the human genome. Nucleic Acids Res 35: 3741–3751.
  71. 71. Orii KE, Lee Y, Kondo N, McKinnon PJ (2006) Selective utilization of nonhomologous end-joining and homologous recombination DNA repair pathways during nervous system development. Proc Natl Acad Sci U S A 103: 10017–10022.
  72. 72. Preston CR, Flores CC, Engels WR (2006) Differential usage of alternative pathways of double-strand break repair in Drosophila. Genetics 172: 1055–1068.
  73. 73. Morrish TA, Gilbert N, Myers JS, Vincent BJ, Stamato TD, et al. (2002) DNA repair mediated by endonuclease-independent LINE-1 retrotransposition. Nat Genet 31: 159–165.
  74. 74. Karolchik D, Hinrichs AS, Furey TS, Roskin KM, Sugnet CW, et al. (2004) The UCSC Table Browser data retrieval tool. Nucleic Acids Res 32 Database issue: D493–496.
  75. 75. Rubnitz J, Subramani S (1984) The minimum amount of homology required for homologous recombination in mammalian cells. Mol Cell Biol 4: 2253–2258.
  76. 76. Swofford DL (1995) PAUP*. Sunderland, Massachusetts: Phylogenetic Analysis Using Parsimony (* and Other Methods) Sinauer Associates.
  77. 77. Higgins DG, Thompson JD, Gibson TJ (1996) Using CLUSTAL for multiple sequence alignments. Methods Enzymol 266: 383–402.