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Public Library of Science

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Issue Image

PLoS Genetics Issue Image | Vol. 4(12) December 2008

<b>Expression of the eGFP::I-PpoI fusion protein in <i>Anopheles gambiae</i> testes.</b>

Pictured is a confocal image of a mosquito testicle stained for DNA (with DAPI, shown in red) overlaid with the expression pattern of the eGFP-tagged I-PpoI endonuclease protein (shown in green). The testis hub region (lower right part of the picture) does not stain for eGFP, suggesting that stem cells are not affected by the potentially toxic endonuclease. Expression of the protein during later stages of spermatogenesis induced dominant male sterility and sex chromosome transmission ratio distortion, without disrupting the production of functional sperm (see Windbichler et al., doi:10.1371/journal.pgen.1000291).

Image Credit: Image generated by Philippos Aris Papathanos (Imperial College, London).

Expression of the eGFP::I-PpoI fusion protein in Anopheles gambiae testes. Top

Pictured is a confocal image of a mosquito testicle stained for DNA (with DAPI, shown in red) overlaid with the expression pattern of the eGFP-tagged I-PpoI endonuclease protein (shown in green). The testis hub region (lower right part of the picture) does not stain for eGFP, suggesting that stem cells are not affected by the potentially toxic endonuclease. Expression of the protein during later stages of spermatogenesis induced dominant male sterility and sex chromosome transmission ratio distortion, without disrupting the production of functional sperm (see Windbichler et al., doi:10.1371/journal.pgen.1000291).

Image Credit: Image generated by Philippos Aris Papathanos (Imperial College, London).

Citation: (2008) PLoS Genetics Issue Image | Vol. 4(12) December 2008. PLoS Genet 4(12): ev04.i12. doi:10.1371/image.pgen.v04.i12

Published: December 26, 2008

Copyright: © 2008 Windbichler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Expression of the eGFP::I-PpoI fusion protein in Anopheles gambiae testes.

Pictured is a confocal image of a mosquito testicle stained for DNA (with DAPI, shown in red) overlaid with the expression pattern of the eGFP-tagged I-PpoI endonuclease protein (shown in green). The testis hub region (lower right part of the picture) does not stain for eGFP, suggesting that stem cells are not affected by the potentially toxic endonuclease. Expression of the protein during later stages of spermatogenesis induced dominant male sterility and sex chromosome transmission ratio distortion, without disrupting the production of functional sperm (see Windbichler et al., doi:10.1371/journal.pgen.1000291).

Image Credit: Image generated by Philippos Aris Papathanos (Imperial College, London).

doi:10.1371/image.pgen.v04.i12.g001
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