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Research Article

miR-199a-5p Is Upregulated during Fibrogenic Response to Tissue Injury and Mediates TGFbeta-Induced Lung Fibroblast Activation by Targeting Caveolin-1

  • Christian Lacks Lino Cardenas equal contributor,

    equal contributor Contributed equally to this work with: Christian Lacks Lino Cardenas, Imène Sarah Henaoui, Bernard Mari, Nicolas Pottier

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Imène Sarah Henaoui equal contributor,

    equal contributor Contributed equally to this work with: Christian Lacks Lino Cardenas, Imène Sarah Henaoui, Bernard Mari, Nicolas Pottier

    Affiliations: Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France, Université de Nice Sophia-Antipolis, Nice, France

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  • Elisabeth Courcot,

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Christoph Roderburg,

    Affiliation: Department of Medicine III, University Hospital RWTH Aachen, Aachen, Germany

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  • Christelle Cauffiez,

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Sébastien Aubert,

    Affiliations: Institut National de la Santé et de la Recherche Médicale, U837, Jean-Pierre Aubert Research Center, Equipe 5 “Mucines, Différentiation et Cancérogenèse Épithéliales”, Lille, France, Pôle de Pathologie, CHRU Lille, Lille, France, Faculté de Médecine, Université de Lille 2, Lille, France

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  • Marie-Christine Copin,

    Affiliations: Institut National de la Santé et de la Recherche Médicale, U837, Jean-Pierre Aubert Research Center, Equipe 5 “Mucines, Différentiation et Cancérogenèse Épithéliales”, Lille, France, Pôle de Pathologie, CHRU Lille, Lille, France, Faculté de Médecine, Université de Lille 2, Lille, France

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  • Benoit Wallaert,

    Affiliation: Service de Pneumologie et Immunoallergologie, CHRU Lille, Lille, France

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  • François Glowacki,

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Edmone Dewaeles,

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Jadranka Milosevic,

    Affiliation: Dorothy P. and Richard P. Simmons Center for Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America

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  • Julien Maurizio,

    Affiliations: Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France, Université de Nice Sophia-Antipolis, Nice, France

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  • John Tedrow,

    Affiliation: Dorothy P. and Richard P. Simmons Center for Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America

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  • Brice Marcet,

    Affiliations: Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France, Université de Nice Sophia-Antipolis, Nice, France

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  • Jean-Marc Lo-Guidice,

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Naftali Kaminski,

    Affiliation: Dorothy P. and Richard P. Simmons Center for Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America

    X
  • Pascal Barbry,

    Affiliations: Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France, Université de Nice Sophia-Antipolis, Nice, France

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  • Tom Luedde,

    Affiliation: Department of Medicine III, University Hospital RWTH Aachen, Aachen, Germany

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  • Michael Perrais,

    Affiliations: Institut National de la Santé et de la Recherche Médicale, U837, Jean-Pierre Aubert Research Center, Equipe 5 “Mucines, Différentiation et Cancérogenèse Épithéliales”, Lille, France, Faculté de Médecine, Université de Lille 2, Lille, France

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  • Bernard Mari equal contributor mail,

    equal contributor Contributed equally to this work with: Christian Lacks Lino Cardenas, Imène Sarah Henaoui, Bernard Mari, Nicolas Pottier

    mari@unice.fr (B Mari) (BM); nico_pottier@yahoo.fr (N Pottier) (NP)

    Affiliations: Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France, Université de Nice Sophia-Antipolis, Nice, France

    X
  • Nicolas Pottier equal contributor mail

    equal contributor Contributed equally to this work with: Christian Lacks Lino Cardenas, Imène Sarah Henaoui, Bernard Mari, Nicolas Pottier

    mari@unice.fr (B Mari) (BM); nico_pottier@yahoo.fr (N Pottier) (NP)

    Affiliation: EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France

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  • Published: February 14, 2013
  • DOI: 10.1371/journal.pgen.1003291

About the Authors

Christian Lacks Lino Cardenas, Elisabeth Courcot, Christelle Cauffiez, François Glowacki, Edmone Dewaeles, Jean-Marc Lo-Guidice, Nicolas Pottier
EA4483, Faculté de Médecine de Lille, Pole Recherche, Lille, France
Imène Sarah Henaoui, Julien Maurizio, Brice Marcet, Pascal Barbry, Bernard Mari
Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR-7275, Valbonne Sophia-Antipolis, France
Imène Sarah Henaoui, Julien Maurizio, Brice Marcet, Pascal Barbry, Bernard Mari
Université de Nice Sophia-Antipolis, Nice, France
Christoph Roderburg, Tom Luedde
Department of Medicine III, University Hospital RWTH Aachen, Aachen, Germany
Sébastien Aubert, Marie-Christine Copin, Michael Perrais
Institut National de la Santé et de la Recherche Médicale, U837, Jean-Pierre Aubert Research Center, Equipe 5 “Mucines, Différentiation et Cancérogenèse Épithéliales”, Lille, France
Sébastien Aubert, Marie-Christine Copin
Pôle de Pathologie, CHRU Lille, Lille, France
Sébastien Aubert, Marie-Christine Copin, Michael Perrais
Faculté de Médecine, Université de Lille 2, Lille, France
Benoit Wallaert
Service de Pneumologie et Immunoallergologie, CHRU Lille, Lille, France
Jadranka Milosevic, John Tedrow, Naftali Kaminski
Dorothy P. and Richard P. Simmons Center for Interstitial Lung Disease, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America

Corresponding Authors

Competing Interests

N Kaminski has a patent application for use of microRNAs as therapeutic targets in Idiopathic Pulmonary Fibrosis.

Author Contributions

Conceived and designed the experiments: N Pottier, B Mari, M Perrais, C Cauffiez, T Luedde, N Kaminski, C Roderburg. Performed the experiments: IS Henaoui, N Pottier, C Roderburg, S Aubert, E Courcot, J Milosevic, B Marcet, CL Lino Cardenas, F Glowacki, J-M Lo-Guidice, E Dewaeles. Analyzed the data: N Pottier, B Mari, P Barbry, N Kaminski, T Luedde, C Cauffiez, M Perrais, J Tedrow, J Milosevic, J Maurizio, M-C Copin. Contributed reagents/materials/analysis tools: B Wallaert, M-C Copin. Wrote the paper: N Pottier, B Mari, P Barbry, N Kaminski.