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Research Article

Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

  • Timothy J. Dahlem equal contributor,

    equal contributor Contributed equally to this work with: Timothy J. Dahlem, Kazuyuki Hoshijima

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Kazuyuki Hoshijima equal contributor,

    equal contributor Contributed equally to this work with: Timothy J. Dahlem, Kazuyuki Hoshijima

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Michael J. Jurynec,

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Derrick Gunther,

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Colby G. Starker,

    Affiliation: Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Alexandra S. Locke,

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Allison M. Weis,

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Daniel F. Voytas,

    Affiliation: Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • David Jonah Grunwald mail

    grunwald@genetics.utah.edu

    Affiliation: Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America

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  • Published: August 16, 2012
  • DOI: 10.1371/journal.pgen.1002861

About the Authors

Timothy J. Dahlem, Kazuyuki Hoshijima, Michael J. Jurynec, Derrick Gunther, Alexandra S. Locke, Allison M. Weis, David Jonah Grunwald
Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America
Colby G. Starker, Daniel F. Voytas
Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America

Corresponding Author

Email: grunwald@genetics.utah.edu

Competing Interests

DFV is a listed inventor on a patent application titled "TAL effector-mediated DNA modification" that is co-owned by Iowa State University and the University of Minnesota and that has been licensed to Cellectis, a European biotechnology company. All other authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TJD KH MJJ DJG. Performed the experiments: TJD KH MJJ DG ASL AMW DJG. Analyzed the data: TJD KH MJJ DG ASL AMW DJG. Contributed reagents/materials/analysis tools: CGS DFV. Wrote the paper: DJG KH TJD MJJ.