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Research Article

Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity

  • Mitsuhiro Endoh mail,

    mendoh@rcai.riken.jp (ME); koseki@rcai.riken.jp (HK)

    Affiliations: Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Yokohama, Japan

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  • Takaho A. Endo equal contributor,

    equal contributor Contributed equally to this work with: Takaho A. Endo, Tamie Endoh

    Affiliation: RIKEN Bioinformatics and System Engineering Division, Yokohama, Japan

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  • Tamie Endoh equal contributor,

    equal contributor Contributed equally to this work with: Takaho A. Endo, Tamie Endoh

    Affiliation: Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan

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  • Kyo-ichi Isono,

    Affiliation: Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan

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  • Jafar Sharif,

    Affiliation: Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan

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  • Osamu Ohara,

    Affiliation: Laboratories for Immunogenomics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan

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  • Tetsuro Toyoda,

    Affiliation: RIKEN Bioinformatics and System Engineering Division, Yokohama, Japan

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  • Takashi Ito,

    Affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, Japan

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  • Ragnhild Eskeland,

    Affiliation: MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

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  • Wendy A. Bickmore,

    Affiliation: MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

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  • Miguel Vidal,

    Affiliations: Cell Proliferation and Development, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain, Research Unit for Immunoepigenetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan

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  • Bradley E. Bernstein,

    Affiliations: Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America, Department of Pathology, Harvard Medical School, Boston, Massachusetts, United States of America, Broad Institute of Harvard and MIT, Cambridge, Massachusetts, United States of America

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  • Haruhiko Koseki mail

    mendoh@rcai.riken.jp (ME); koseki@rcai.riken.jp (HK)

    Affiliations: Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Yokohama, Japan

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  • Published: July 26, 2012
  • DOI: 10.1371/journal.pgen.1002774

About the Authors

Mitsuhiro Endoh, Tamie Endoh, Kyo-ichi Isono, Jafar Sharif, Haruhiko Koseki
Laboratory for Developmental Genetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
Mitsuhiro Endoh, Haruhiko Koseki
Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Yokohama, Japan
Takaho A. Endo, Tetsuro Toyoda
RIKEN Bioinformatics and System Engineering Division, Yokohama, Japan
Osamu Ohara
Laboratories for Immunogenomics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
Takashi Ito
Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, Japan
Ragnhild Eskeland, Wendy A. Bickmore
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom
Miguel Vidal
Cell Proliferation and Development, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain
Miguel Vidal
Research Unit for Immunoepigenetics, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
Bradley E. Bernstein
Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
Bradley E. Bernstein
Department of Pathology, Harvard Medical School, Boston, Massachusetts, United States of America
Bradley E. Bernstein
Broad Institute of Harvard and MIT, Cambridge, Massachusetts, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: ME HK. Performed the experiments: ME TE K-iI. Analyzed the data: TAE OO TT. Contributed reagents/materials/analysis tools: TI RE WAB MV. Wrote the paper: ME JS WAB BEB HK.