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Research Article

Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways

  • Mohan Babu equal contributor mail,

    equal contributor Contributed equally to this work with: Mohan Babu, J. Javier Díaz-Mejía

    mohan.babu@utoronto.ca (MB); andrew.emili@utoronto.ca (AE)

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • J. Javier Díaz-Mejía equal contributor,

    equal contributor Contributed equally to this work with: Mohan Babu, J. Javier Díaz-Mejía

    Affiliations: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada, Department of Biology, Wilfrid Laurier University, Waterloo, Canada

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  • James Vlasblom,

    Affiliations: Hospital for Sick Children, Toronto, Canada, Department of Biochemistry, University of Toronto, Toronto, Canada

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  • Alla Gagarinova,

    Affiliations: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Canada

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  • Sadhna Phanse,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Chris Graham,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Fouad Yousif,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Huiming Ding,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Xuejian Xiong,

    Affiliation: Hospital for Sick Children, Toronto, Canada

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  • Anaies Nazarians-Armavil,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Md Alamgir,

    Affiliation: Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Canada

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  • Mehrab Ali,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Oxana Pogoutse,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Asaf Pe'er,

    Affiliation: Department of Microbiology and Molecular Genetics, Institute for Medical Research-Israel-Canada, The Hebrew University of Jerusalem, Jerusalem, Israel

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  • Roland Arnold,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • Magali Michaut,

    Affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada

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  • John Parkinson,

    Affiliations: Hospital for Sick Children, Toronto, Canada, Department of Biochemistry, University of Toronto, Toronto, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Canada

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  • Ashkan Golshani,

    Affiliation: Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Canada

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  • Chris Whitfield,

    Affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada

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  • Shoshana J. Wodak,

    Affiliations: Hospital for Sick Children, Toronto, Canada, Department of Biochemistry, University of Toronto, Toronto, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Canada

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  • Gabriel Moreno-Hagelsieb,

    Affiliation: Department of Biology, Wilfrid Laurier University, Waterloo, Canada

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  • Jack F. Greenblatt,

    Affiliations: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Canada

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  • Andrew Emili mail

    mohan.babu@utoronto.ca (MB); andrew.emili@utoronto.ca (AE)

    Affiliations: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Canada

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  • Published: November 17, 2011
  • DOI: 10.1371/journal.pgen.1002377

About the Authors

Mohan Babu, J. Javier Díaz-Mejía, Alla Gagarinova, Sadhna Phanse, Chris Graham, Fouad Yousif, Huiming Ding, Anaies Nazarians-Armavil, Mehrab Ali, Oxana Pogoutse, Roland Arnold, Magali Michaut, Jack F. Greenblatt, Andrew Emili
Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Canada
J. Javier Díaz-Mejía, Gabriel Moreno-Hagelsieb
Department of Biology, Wilfrid Laurier University, Waterloo, Canada
James Vlasblom, Xuejian Xiong, John Parkinson, Shoshana J. Wodak
Hospital for Sick Children, Toronto, Canada
James Vlasblom, John Parkinson, Shoshana J. Wodak
Department of Biochemistry, University of Toronto, Toronto, Canada
Alla Gagarinova, John Parkinson, Shoshana J. Wodak, Jack F. Greenblatt, Andrew Emili
Department of Molecular Genetics, University of Toronto, Toronto, Canada
Md Alamgir, Ashkan Golshani
Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Canada
Asaf Pe'er
Department of Microbiology and Molecular Genetics, Institute for Medical Research-Israel-Canada, The Hebrew University of Jerusalem, Jerusalem, Israel
Chris Whitfield
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: M Babu, JJ Díaz-Mejía, A Emili. Performed the experiments: M Babu, A Gagarinova, C Graham, A Nazarians-Armavil, M Alamgir, M Ali, O Pogoutse, A Golshani. Analyzed the data: M Babu, JJ Díaz-Mejía, J Vlasblom, S Phanse, F Yousif, H Ding, X Xiong, A Pe'er, R Arnold, M Michaut, G Moreno-Hagelsieb. Contributed reagents/materials/analysis tools: J Parkinson, SJ Wodak, C Whitfield, JF Greenblatt. Wrote the paper: M Babu, A Emili. Project conceived by: G Moreno-Hagelsieb, JF Greenblatt, A Emili. Contributed to the text: JJ Díaz-Mejía, J Vlasblom, A Gagarinova, J Parkinson, SJ Wodak, C Whitfield, JF Greenblatt.