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Research Article

Identification of Small Molecule Inhibitors of Pseudomonas aeruginosa Exoenzyme S Using a Yeast Phenotypic Screen

  • Anthony Arnoldo,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Jasna Curak,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Saranya Kittanakom,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Igor Chevelev,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Vincent T. Lee,

    Affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Mehdi Sahebol-Amri,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Becky Koscik,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Lana Ljuma,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Peter J. Roy,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Antonio Bedalov,

    Affiliation: Clinical Research and Human Biology Divisions, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Guri Giaever,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada, Department of Pharmaceutical Sciences, University of Toronto, Ontario, Canada

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  • Corey Nislow,

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada, Department of Pharmaceutical Sciences, University of Toronto, Ontario, Canada

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  • Rod A. Merrill,

    Affiliation: Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada

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  • Stephen Lory,

    Affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Igor Stagljar mail

    igor.stagljar@utoronto.ca

    Affiliations: Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada, Department of Molecular Genetics, University of Toronto, Ontario, Canada

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  • Published: February 29, 2008
  • DOI: 10.1371/journal.pgen.1000005

About the Authors

Anthony Arnoldo, Jasna Curak, Saranya Kittanakom, Igor Chevelev, Mehdi Sahebol-Amri, Becky Koscik, Lana Ljuma, Peter J. Roy, Guri Giaever, Corey Nislow, Igor Stagljar
Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada
Anthony Arnoldo, Jasna Curak, Saranya Kittanakom, Igor Chevelev, Mehdi Sahebol-Amri, Becky Koscik, Lana Ljuma, Peter J. Roy, Guri Giaever, Corey Nislow, Igor Stagljar
Department of Molecular Genetics, University of Toronto, Ontario, Canada
Vincent T. Lee, Stephen Lory
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
Antonio Bedalov
Clinical Research and Human Biology Divisions, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
Guri Giaever, Corey Nislow
Department of Pharmaceutical Sciences, University of Toronto, Ontario, Canada
Rod A. Merrill
Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada

Corresponding Author

Email: igor.stagljar@utoronto.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AA IS. Performed the experiments: AA JC SK IC VL MS BK LL RM. Analyzed the data: AA SK RM. Contributed reagents/materials/analysis tools: AA PR AB GG CN SL. Wrote the paper: AA SK CN.