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Research Article

Subfunctionalization of Duplicated Zebrafish pax6 Genes by cis-Regulatory Divergence

  • Dirk A Kleinjan equal contributor,

    equal contributor Contributed equally to this work with: Dirk A Kleinjan, Ruth M Bancewicz

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Ruth M Bancewicz equal contributor,

    equal contributor Contributed equally to this work with: Dirk A Kleinjan, Ruth M Bancewicz

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

    ¤a Current address: The Faraday Institute for Science and Religion, St Edmund's College, Cambridge, UK

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  • Philippe Gautier,

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Ralf Dahm,

    Affiliation: Department of Genetics, Max-Planck Institute for Developmental Biology, Tübingen, Germany

    ¤b Current address: Center for Brain Research, Medical University of Vienna, Vienna, Austria

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  • Helia B Schonthaler,

    Affiliation: Department of Genetics, Max-Planck Institute for Developmental Biology, Tübingen, Germany

    ¤c Current address: Research Institute of Molecular Pathology, Vienna, Austria

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  • Giuseppe Damante,

    Affiliation: Department of Science and Biomedical Technology, University of Udine, Udine, Italy

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  • Anne Seawright,

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Ann M Hever,

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Patricia L Yeyati,

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Veronica van Heyningen mail,

    To whom correspondence should be addressed. E-mail: v.vanheyningen@hgu.mrc.ac.uk

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Pedro Coutinho

    Affiliation: Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom

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  • Published: February 15, 2008
  • DOI: 10.1371/journal.pgen.0040029

About the Authors

Dirk A Kleinjan, Ruth M Bancewicz, Philippe Gautier, Anne Seawright, Ann M Hever, Patricia L Yeyati, Veronica van Heyningen, Pedro Coutinho
Medical Research Council (MRC) Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom
Ralf Dahm, Helia B Schonthaler
Department of Genetics, Max-Planck Institute for Developmental Biology, Tübingen, Germany
Giuseppe Damante
Department of Science and Biomedical Technology, University of Udine, Udine, Italy

Corresponding Author

Email: v.vanheyningen@hgu.mrc.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

DAK, RMB, VvH, and PC conceived and designed the experiments and analyzed the data with significant input by PLY. RMB carried out the mating and phenotype selection and confirmations for the mapping. DAK and PC developed the transgenic strategies and constructs and made and analyzed mouse and fish transgenics with help from AS. PC carried out mRNA rescue and morpholino experiments. PG and PC provided bioinformatics contributions. RD supplied the sunrise mutant and worked with HBS on the fish mapping. RD and PLY analyzed the fish eye phenotype. AMH analyzed the pancreas. GD conceived and carried out the mutant protein functional analyses. VvH wrote the paper.