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Research Article

A Screen for Suppressors of Gross Chromosomal Rearrangements Identifies a Conserved Role for PLP in Preventing DNA Lesions

  • Pamela Kanellis,

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada

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  • Mark Gagliardi,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Judit P Banath,

    Affiliation: British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

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  • Rachel K Szilard,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Shinichiro Nakada,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Sarah Galicia,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Frederic D Sweeney,

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada

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  • Diane C Cabelof,

    Affiliation: Karmanos Cancer Institute, Detroit, Michigan, United States of America

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  • Peggy L Olive,

    Affiliation: British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

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  • Daniel Durocher mail

    To whom correspondence should be addressed. E-mail: durocher@mshri.on.ca

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada

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  • Published: August 10, 2007
  • DOI: 10.1371/journal.pgen.0030134

About the Authors

Pamela Kanellis, Mark Gagliardi, Rachel K Szilard, Shinichiro Nakada, Sarah Galicia, Frederic D Sweeney, Daniel Durocher
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Pamela Kanellis, Frederic D Sweeney, Daniel Durocher
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada
Judit P Banath, Peggy L Olive
British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada
Diane C Cabelof
Karmanos Cancer Institute, Detroit, Michigan, United States of America

Corresponding Author

Email: durocher@mshri.on.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

PK and DD conceived and designed the experiments, except for the FACS analysis of γ-H2AX, which was designed by PLO, and uracil quantitation, which was designed with DCC. PK performed all the yeast-related experiments except for the examination of Rad53 activity, which was done with the help of FDS. MG examined the formation of 53BP1 foci in response to 4-DP and examined the activation of the checkpoint response by Western blotting. JB quantitated γ-H2AX formation by flow cytometry. RKS mapped the breakpoints by CGH. SN examined the levels of apoptosis in response to 4-DP. SG performed some of the GCR assays. DCC determined uracil content in genomic DNA. PK and DD wrote the paper with valued input from RKS, DCC, and PLO.