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Research Article

Harnessing a High Cargo-Capacity Transposon for Genetic Applications in Vertebrates

  • Darius Balciunas,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Kirk J Wangensteen equal contributor,

    equal contributor Contributed equally to this work with: Kirk J Wangensteen, Andrew Wilber

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America

    X
  • Andrew Wilber equal contributor,

    equal contributor Contributed equally to this work with: Kirk J Wangensteen, Andrew Wilber

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Gene Therapy Program, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Jason Bell,

    Affiliation: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Aron Geurts,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

    X
  • Sridhar Sivasubbu,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Xin Wang,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Perry B Hackett,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Gene Therapy Program, University of Minnesota, Minneapolis, Minnesota, United States of America, Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • David A Largaespada,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • R. Scott McIvor,

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Gene Therapy Program, University of Minnesota, Minneapolis, Minnesota, United States of America, Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Stephen C Ekker mail

    To whom correspondence should be addressed. E-mail: ekker001@umn.edu

    Affiliations: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America, Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America, Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Published: November 10, 2006
  • DOI: 10.1371/journal.pgen.0020169

About the Authors

Darius Balciunas, Kirk J Wangensteen, Andrew Wilber, Jason Bell, Aron Geurts, Sridhar Sivasubbu, Xin Wang, Perry B Hackett, David A Largaespada, R. Scott McIvor, Stephen C Ekker
The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America
Darius Balciunas, Aron Geurts, Sridhar Sivasubbu, Perry B Hackett, David A Largaespada, R. Scott McIvor, Stephen C Ekker
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America
Kirk J Wangensteen, Stephen C Ekker
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America
Andrew Wilber, Perry B Hackett, R. Scott McIvor
Gene Therapy Program, University of Minnesota, Minneapolis, Minnesota, United States of America
Aron Geurts, David A Largaespada, R. Scott McIvor, Stephen C Ekker
Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America
Xin Wang, Perry B Hackett, Stephen C Ekker
Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, United States of America

Corresponding Author

Email: ekker001@umn.edu

Competing Interests

We note that the University of Minnesota and PBH, RSM, DAL, and SCE are all founders of a gene therapy company called Discovery Genomics, Incorporated.

Author Contributions

DB, KJW, AW, JB, AG, SS, PBH, DAL, RSM, and SCE conceived and designed the experiments. DB, KJW, AW, JB, AG, and SS performed the experiments. DB, KJW, AW, JB, AG, SS, PBH, DAL, RSM, and SCE analyzed the data. DB, KJW, AW, JB, AG, XW, and DAL contributed reagents/materials/analysis tools. DB, KJW, AW, PBH, RSM, and SCE wrote the paper.