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Research Article

Exploring the Mycobacteriophage Metaproteome: Phage Genomics as an Educational Platform

  • Graham F Hatfull mail,

    To whom correspondence should be addressed. E-mail: gfh@pitt.edu

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    X
  • Marisa L Pedulla,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤a Current address: Biology Department, Montana Tech of the University of Montana, Butte, Montana, United States of America

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  • Deborah Jacobs-Sera,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Pauline M Cichon,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Amy Foley,

    Affiliation: Hampton High School, Allison Park, Pennsylvania, United States of America

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  • Michael E Ford,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤b Current address: J. Weill Medical College of Cornell University, New York, New York, United States of America

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  • Rebecca M Gonda,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Jennifer M Houtz,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Andrew J Hryckowian,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Vanessa A Kelchner,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤c Current address: Bristol-Myers Squibb, Pennington, New Jersey, United States of America

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  • Swathi Namburi,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Kostandin V Pajcini,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤d Current address: Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America

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  • Mark G Popovich,

    Affiliation: Lincoln High School, Elwood City, Pennsylvania, United States of America

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  • Donald T Schleicher,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Brian Z Simanek,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤e Current address: Department of Mathematics and Statistics, Williams College, Bronfman Science Center, Williamstown, Massachusetts, United States of America

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  • Alexis L Smith,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Gina M Zdanowicz,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

    ¤f Current address: Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Vanaja Kumar,

    Affiliation: Tuberculosis Research Center, ICMR, Chetpet, Chennai, India

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  • Craig L Peebles,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • William R Jacobs Jr.,

    Affiliations: Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, New York, United States of America, Howard Hughes Medical Institute, Albert Einstein College of Medicine, New York, New York, United States of America

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  • Jeffrey G Lawrence,

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Roger W Hendrix

    Affiliations: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America

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  • Published: June 09, 2006
  • DOI: 10.1371/journal.pgen.0020092

About the Authors

Graham F Hatfull, Marisa L Pedulla, Deborah Jacobs-Sera, Pauline M Cichon, Michael E Ford, Rebecca M Gonda, Jennifer M Houtz, Andrew J Hryckowian, Vanessa A Kelchner, Swathi Namburi, Kostandin V Pajcini, Donald T Schleicher, Brian Z Simanek, Alexis L Smith, Gina M Zdanowicz, Craig L Peebles, Jeffrey G Lawrence, Roger W Hendrix
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
Graham F Hatfull, Marisa L Pedulla, Deborah Jacobs-Sera, Pauline M Cichon, Michael E Ford, Rebecca M Gonda, Jennifer M Houtz, Andrew J Hryckowian, Vanessa A Kelchner, Swathi Namburi, Kostandin V Pajcini, Donald T Schleicher, Brian Z Simanek, Alexis L Smith, Gina M Zdanowicz, Craig L Peebles, Jeffrey G Lawrence, Roger W Hendrix
Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
Amy Foley
Hampton High School, Allison Park, Pennsylvania, United States of America
Mark G Popovich
Lincoln High School, Elwood City, Pennsylvania, United States of America
Vanaja Kumar
Tuberculosis Research Center, ICMR, Chetpet, Chennai, India
William R Jacobs Jr.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, New York, United States of America
William R Jacobs Jr.
Howard Hughes Medical Institute, Albert Einstein College of Medicine, New York, New York, United States of America

Corresponding Author

Email: gfh@pitt.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

GFH, WRJ, JGL, and RWH conceived and designed the experiments. MLP, PMC, AF, MEF, RMG, JMH, AJH, VAK, SN, KVP, MGP, DTS, BZS, ALS, and GMZ performed the experiments. GFH, MLP, DJS, PMC, AF, MEF, RMG, JMH, AJH, VAK, SN, KVP, MGP, DTS, BZS, ALS, GMZ, VK, CLP, JGL, and RWH analyzed the data. PMC, AF, RMG, JMH, AJH, VAK, SN, KVP, MGP, DTS, BZS, ALS, GMZ, VK, and JGL contributed reagents/materials/analysis tools. GFH, MLP, DJS, CLP, WRJ, JGL, and RWH wrote the paper.