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Research Article

A Combination of H2A.Z and H4 Acetylation Recruits Brd2 to Chromatin during Transcriptional Activation

  • Ryan Draker,

    Affiliations: Ontario Cancer Institute, Toronto, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Canada

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  • Marlee K. Ng,

    Affiliation: Department of Biology, York University, Toronto, Canada

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  • Elizabeth Sarcinella,

    Affiliation: Ontario Cancer Institute, Toronto, Canada

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  • Vladimir Ignatchenko,

    Affiliation: Ontario Cancer Institute, Toronto, Canada

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  • Thomas Kislinger,

    Affiliations: Ontario Cancer Institute, Toronto, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Canada

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  • Peter Cheung mail

    pmcheung@yorku.ca

    Affiliations: Ontario Cancer Institute, Toronto, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Canada, Department of Biology, York University, Toronto, Canada

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  • Published: November 08, 2012
  • DOI: 10.1371/journal.pgen.1003047

Reader Comments (1)

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Peptide sequences please

Posted by ruski09 on 14 Nov 2012 at 17:47 GMT

Good study and quite challenging from the methodology point of view. Would have liked to see the sequences of the peptides (i.e. the lengths of the peptides) used in the peptide competition experiments though.

No competing interests declared.

RE: Peptide sequences please

petcheung replied to ruski09 on 14 Nov 2012 at 21:01 GMT

Thank you for your interest in our paper and work.

3 series of peptides were used in the competition assay shown in Fig 3B.

For the H4 peptides, they correspond to amino acids 1 - 20 of the human H4 sequence, with or without acetylation at K12, or K5/K8/K12/K16 (tetra).

For the H2A.Z peptides, they correspond to amino acids 1 - 16 of the human H2A.Z sequence, with or without acetylation at the sites indicated in the figure.

Finally, for the H2A peptides, they correspond to amino acids 1 - 14 of the human H2A sequence, with or without acetylation at the indicated sites.

I hope this answers your question.

Best regards,

Peter

No competing interests declared.