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Research Article

TCF7L2 Modulates Glucose Homeostasis by Regulating CREB- and FoxO1-Dependent Transcriptional Pathway in the Liver

  • Kyoung-Jin Oh,

    Affiliation: Division of Biochemistry and Molecular Biology, Department of Molecular Cell Biology and Samsung Biomedical Institute, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi-do, Korea

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  • Jinyoung Park,

    Affiliation: Division of Biochemistry and Molecular Biology, Department of Molecular Cell Biology and Samsung Biomedical Institute, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi-do, Korea

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  • Su Sung Kim,

    Affiliation: Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, Gil Medical Center, Gachon University, Yeonsu-ku, Incheon, Korea

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  • Hyunhee Oh,

    Affiliation: Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, Gil Medical Center, Gachon University, Yeonsu-ku, Incheon, Korea

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  • Cheol Soo Choi,

    Affiliation: Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, Gil Medical Center, Gachon University, Yeonsu-ku, Incheon, Korea

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  • Seung-Hoi Koo mail

    shkoo@skku.edu

    Affiliation: Division of Biochemistry and Molecular Biology, Department of Molecular Cell Biology and Samsung Biomedical Institute, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi-do, Korea

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  • Published: September 27, 2012
  • DOI: 10.1371/journal.pgen.1002986

Reader Comments (2)

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Which TCF7L2 mouse was used here?

Posted by SSamson on 13 Oct 2012 at 19:46 GMT

The original TCF7l2 mouse available from Eucomm was made from EPD0130_2_C06 ES clone. This mouse is now known to have an insertion event and duplication of the critical exon.

No competing interests declared.

RE: Which TCF7L2 mouse was used here?

koohoi replied to SSamson on 12 Jun 2013 at 07:29 GMT

The original TCF7L2 mice from Eucomm did have an insertion of targeting vector, rather than the recombination. Thus we were able to use the line with knockout-first purposes (for general knockout-in this case het only since homozygotes are lethal) only. We used the knockout-first line for the study. However, we were not able to generate correct loxP/loxP mice using Flp mice (this will lead to the formation of chimeric gene, which would probably encode a trucated protein at best) from this line. They have corrected the problem since, and provided us the new line (from EPD0157_5_B10 clone) this year. Hopefully, we could generate the conditional KO mice from the new line.

No competing interests declared.