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Research Article

A Myo6 Mutation Destroys Coordination between the Myosin Heads, Revealing New Functions of Myosin VI in the Stereocilia of Mammalian Inner Ear Hair Cells

  • Ronna Hertzano equal contributor,

    equal contributor Contributed equally to this work with: Ronna Hertzano, Ella Shalit, Agnieszka K. Rzadzinska

    Affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel

    Current address: Department of Otorhinolaryngology Head and Neck Surgery, University of Maryland, Baltimore, Maryland, United States of America

    X
  • Ella Shalit equal contributor,

    equal contributor Contributed equally to this work with: Ronna Hertzano, Ella Shalit, Agnieszka K. Rzadzinska

    Affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel

    X
  • Agnieszka K. Rzadzinska equal contributor,

    equal contributor Contributed equally to this work with: Ronna Hertzano, Ella Shalit, Agnieszka K. Rzadzinska

    Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom

    X
  • Amiel A. Dror,

    Affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel

    X
  • Lin Song,

    Affiliation: Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

    X
  • Uri Ron,

    Affiliation: Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel

    X
  • Joshua T. Tan,

    Affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California, United States of America

    X
  • Alina Starovolsky Shitrit,

    Affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel

    X
  • Helmut Fuchs,

    Affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Experimental Genetics, Neuherberg, Germany

    X
  • Tama Hasson,

    Affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California, United States of America

    Current address: UCLA College of Letters and Science, Honors and Undergraduate Programs, University of California at Los Angeles, Los Angeles, California, United States of America

    X
  • Nir Ben-Tal,

    Affiliation: Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel

    X
  • H. Lee Sweeney,

    Affiliation: Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

    X
  • Martin Hrabe de Angelis,

    Affiliations: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Experimental Genetics, Neuherberg, Germany, Technical University of Munich, Munich, Germany

    X
  • Karen P. Steel,

    Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom

    X
  • Karen B. Avraham mail

    karena@post.tau.ac.il

    Affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel

    X
  • Published: October 03, 2008
  • DOI: 10.1371/journal.pgen.1000207

Reader Comments (1)

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Myosin 6 is a monomer

Posted by mpeckham on 12 May 2009 at 13:30 GMT

I'm not sure why this mutation would interfere with 'walking' since myosin 6 is a monomer (Buss et al., 2004, Embo J) (Knight et al., 2005, J. Biol. Chem), and (Spink et al., 2008, NSMB).

No competing interests declared.

RE: Myosin 6 is a monomer

Lee_Sweeney replied to mpeckham on 14 Aug 2009 at 22:33 GMT

Myosin VI is a dimer when bound to cargo and likely always functions as a dimer in cells. It is a monomer when inactive.
References to date (more to come soon):

1) Myosin VI Dimerization Triggers an Unfolding of a Three-Helix Bundle in Order to Extend Its Reach.
Mukherjea M, Llinas P, Kim H, Travaglia M, Safer D, Ménétrey J, Franzini-Armstrong C, Selvin PR, Houdusse A, Sweeney HL.
Mol Cell. 2009 Aug 5. [Epub ahead of print]

2) Myosin VI undergoes cargo-mediated dimerization.
Yu C, Feng W, Wei Z, Miyanoiri Y, Wen W, Zhao Y, Zhang M.
Cell. 2009 Aug 7;138(3):537-48.

3) Full-length myosin VI dimerizes and moves processively along actin filaments upon monomer clustering.
Park H, Ramamurthy B, Travaglia M, Safer D, Chen LQ, Franzini-Armstrong C, Selvin PR, Sweeney HL.
Mol Cell. 2006 Feb 3;21(3):331-6.

No competing interests declared.